EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.18 | D177A |
mutant enzyme does not catalyze hydroxylation of phytanoyl-CoA |
658877 |
| 1.14.11.18 | D177A |
no activity with phytanoyl-CoA |
659669 |
| 1.14.11.18 | E197Q |
disruption of the 2-oxoglutarate binding pocket |
674512 |
| 1.14.11.18 | F275S |
places a polar side chain in a hydrophobic pocket and possibly interferes with the overall structure or impair protein folding |
674512 |
| 1.14.11.18 | G204S |
disruption of the 2-oxoglutarate binding pocket, uncouples 2-oxoglutarate and phytanoyl-CoA oxidation |
674512 |
| 1.14.11.18 | G204S |
mutation causes partial uncoupling of 2-oxoglutarate conversion from phytanoyl-CoA oxidation |
658877 |
| 1.14.11.18 | H175A |
mutant enzyme does not catalyze hydroxylation of phytanoyl-CoA |
658877 |
| 1.14.11.18 | H175A |
no activity with phytanoyl-CoA |
659669 |
| 1.14.11.18 | H213A |
insoluble mutant enzyme |
659669 |
| 1.14.11.18 | H259A |
mutation removes two of the hydrogen bonds and probably destabilizes the beta-turn, which in turn destabilizes the core double stranded beta-helix |
674512 |
