EC Number   |
Protein Variants |
Reference |
|---|
   1.2.1.104 | D15A |
variant is neither able to form a complex with the E2 component, nor to produce NADH in the overall assay |
758777 |
| 1.2.1.104 | D289A |
the mutant does not have any detectable activity in PDC assay while its activity in thedecarboxylation reaction measured by 2,6-dichlorophenolindophenol assay does not significantly change |
712786 |
| 1.2.1.104 | D289N |
the mutant does not demonstrate any change in the 2,6-dichlorophenolindophenol assay but its activity is reduced to 67% in PDC assay |
712786 |
| 1.2.1.104 | D413A |
substitutions has no large effects on E3 activity when measured in its free form. However, when reconstituted in the complex, the pyruvate dehydrogenase activity is reduced to 18%. The binding affinities of the mutant to the the di-domain of the E3-binding protein are severely reduced |
758720 |
| 1.2.1.104 | D7A |
variant is neither able to form a complex with the E2 component, nor to produce NADH in the overall assay |
758777 |
| 1.2.1.104 | D9A |
variant is neither able to form a complex with the E2 component, nor to produce NADH in the overall assay |
758777 |
| 1.2.1.104 | E12D |
variant is neither able to form a complex with the E2 component, nor to produce NADH in the overall assay |
758777 |
| 1.2.1.104 | E12Q |
variant is neither able to form a complex with the E2 component, nor to produce NADH in the overall assay |
758777 |
| 1.2.1.104 | E229A |
the mutant does not show any significant changes compared with the wild type subunit E1 activity |
712786 |
| 1.2.1.104 | E229Q |
the mutant does not show any significant changes compared with the wild type subunit E1 activity |
712786 |
