EC Number   |
Protein Variants |
Reference |
|---|
    1.2.5.1 | A467T |
mutant poxB4 is deficient in lipid activation. Mutation is located in the C-terminal half of the gene. The difference between poxB3 and poxB4 is the binding of Triton detergents |
704367 |
| 1.2.5.1 | A533T |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. A533T mobility is similar to wild-type, and slower than Y549Term |
704376 |
| 1.2.5.1 | A553V |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. A553V mobility is similar to wild-type, and slower than Y549Term |
704376 |
| 1.2.5.1 | E564P |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. E564P has the slowest mobilityamong the mutants tested |
704376 |
| 1.2.5.1 | more |
expression of a truncated gene lacking the last 24 amino acids of the C-terminus, thus being closely analogous to the activated species produced in vitro by limited chymotrypsin cleavage. The truncated protein is fully active in vitro in the absence of lipid, and its activity is not further increased by addition of lipid activators. The truncated enzyme fails to bind Triton X-114. Strains producing the truncated protein are devoid of oxidase activity in vivo |
702186 |
| 1.2.5.1 | R572E |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. R572E has the fastest mobility among the mutants tested |
704376 |
| 1.2.5.1 | R572G |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. R572G shows a midway mobility |
704376 |
| 1.2.5.1 | R572K |
in native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. R572K mobility is similar to wild-type, and slower than Y549Term |
704376 |
| 1.2.5.1 | R572Term |
deletion of last amino acid. In native gel electrophoresis, mutant enzymes show differing electrophoretic mobilities. R572Term shows a midway mobility |
704376 |
| 1.2.5.1 | S536P |
mutant poxB3 is deficient in lipid activation but retains full catlytic activity. Mutation is located in the C-terminal half of the gene. The difference between poxB3 and poxB4 is the binding of Triton detergents |
704367 |
