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Results 1 - 8 of 8
EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 1.3.98.7C251A insoluble 760607
Display the reaction diagram Show all sequences 1.3.98.7C269A auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo but incapable of converting MftA to MftA* or MftA** 760607
Display the reaction diagram Show all sequences 1.3.98.7C269A/C323A insoluble 760607
Display the reaction diagram Show all sequences 1.3.98.7C30A/C34A/C37A insoluble 760607
Display the reaction diagram Show all sequences 1.3.98.7C30A/C37A radical S-adenosylmethionine mutant, can neither cleave SAM nor modify MftA 760607
Display the reaction diagram Show all sequences 1.3.98.7C310A/C341A auxiliary [4Fe-4S] cluster II mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** 760607
Display the reaction diagram Show all sequences 1.3.98.7C323A auxiliary [4Fe-4S] cluster I mutant, capable of catalyzing the reductive cleavage of SAM to form dAdo, incapable of converting MftA to MftA* or MftA** 760607
Display the reaction diagram Show all sequences 1.3.98.7more systematical replacement of Cys residues by Ala. The RS KO could neither cleave SAM nor modify MftA, consistent with the successful knockout of the RS cluster. Activity assays for Aux I and Aux II KO's also provided insightful results. Both Aux I and Aux II KO's were capable of catalyzing the reductive cleavage of SAM to form dAdo (Figure 3A), suggesting that the RS cluster remained intact and in an active conformation in the mutated proteins. However, when assayed against MftA, both Aux I and Aux II KO's were incapable of converting MftA to MftA* or MftA** 760607
Results 1 - 8 of 8