EC Number   |
Protein Variants |
Reference |
|---|
    1.5.99.B2 | A167V |
moderate reduction of POX activity |
689987 |
| 1.5.99.B2 | A455S |
slight reduction of POX activity |
689987 |
| 1.5.99.B2 | A472T |
slight reduction of POX activity |
689987 |
| 1.5.99.B2 | D426N |
moderate reduction of POX activity |
689987 |
| 1.5.99.B2 | E64A |
the mutation decreases the catalytic efficiency 27fold |
724329 |
| 1.5.99.B2 | F10E/L12E |
two point mutations in helix alphaA. This helix contains several hydrophobic residues. The recombinant MBP-tagged mutant variant exclusively forms tetramers and exhibits excellent catalytic features over a wide range of temperatures |
743113 |
| 1.5.99.B2 | G63A |
the mutation decreases the catalytic efficiency 140fold |
724329 |
| 1.5.99.B2 | H225A |
the mutant is unstable and precipitates from solution below pH 7.0, decreased activity compared to the wild type enzyme |
686651 |
| 1.5.99.B2 | H225Q |
the mutant displays activity down to solution pH 6.0, increased activity compared to the wild type enzyme |
686651 |
| 1.5.99.B2 | K110A |
site-directed mutagenesis, inactive mutant |
-, 741885 |
