EC Number |
Protein Variants |
Reference |
---|
1.7.1.17 | F117A |
the mutant shows 208% activity with Methyl Red and 176% activity with NADH compared to the wild type enzyme |
765245 |
1.7.1.17 | F161A |
inactive |
765245 |
1.7.1.17 | F162A |
site-directed mutagenesis |
742834 |
1.7.1.17 | G140F |
inactive |
765245 |
1.7.1.17 | G141F |
inactive |
765245 |
1.7.1.17 | H143A |
the mutant shows 19% activity with Methyl Red and 76% activity with NADH compared to the wild type enzyme |
765245 |
1.7.1.17 | more |
deletion of the azoR gene in Escherichia coli strain MG1655 has no strong influence on the 7NCCA reduction of the cells even though the pure enzyme displays a clear nitroreductase activity with this compound |
743185 |
1.7.1.17 | N121Q |
115% of wild-type activity |
-, 744201 |
1.7.1.17 | N96A |
the mutant shows 9% activity with Methyl Red and 37% activity with NADH compared to the wild type enzyme |
765245 |
1.7.1.17 | R139A |
the mutant shows 63% activity with Methyl Red and 78% activity with NADH compared to the wild type enzyme |
765245 |