EC Number   |
Protein Variants |
Reference |
|---|
    2.2.1.9 | E55D |
about 30% decrease in catalytic efficiency |
718915 |
| 2.2.1.9 | F490A |
0.6% of wild-type activity |
720243 |
| 2.2.1.9 | I148L |
strong decrease in catalytic efficiency |
718915 |
| 2.2.1.9 | I489A |
1% of wild-type activity |
720243 |
| 2.2.1.9 | K292Q |
150% increase in catalytic efficiency |
718915 |
| 2.2.1.9 | K299A |
150% of wild-type activity |
720243 |
| 2.2.1.9 | L493A |
80% of wild-type activity |
720243 |
| 2.2.1.9 | N117R/L478T |
the mutant preferentially converts (5S,6S)-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylate with a more than 70fold higher ratio than that for the wild type enzyme |
756458 |
| 2.2.1.9 | Q118A |
the mutant is completely inactive |
756007 |
| 2.2.1.9 | R106A |
mutation in the second subunit forming the active site, 10% of wild-type activity |
720243 |
