EC Number |
Protein Variants |
Reference |
---|
2.4.1.288 | D256A |
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ |
-, 718920 |
2.4.1.288 | D256E |
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+ |
-, 718920 |
2.4.1.288 | D258A |
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+ |
-, 718920 |
2.4.1.288 | D258E |
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme |
-, 718920 |
2.4.1.288 | D371A |
inactive |
-, 718920 |
2.4.1.288 | D372A |
inactive |
718920 |
2.4.1.288 | D372E |
inactive |
718920 |
2.4.1.288 | D372S |
the mutant shows no detectable activity |
720037 |
2.4.1.288 | E300S |
the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme |
720037 |
2.4.1.288 | E371S |
the mutant shows no detectable activity |
720037 |