EC Number   |
Protein Variants |
Reference |
|---|
    2.7.7.60 | D106A |
no activity in crude cell free extract |
661089 |
| 2.7.7.60 | D106E |
strongly reduced but detectable activity |
661089 |
| 2.7.7.60 | D106N |
no activity in crude cell free extract |
661089 |
| 2.7.7.60 | D152A |
catalytically inactive |
672138 |
| 2.7.7.60 | E688Q |
site-directed mutagenesis, the mutant shows reduced sensitivity to inhibition by MMV008138 as compared to the wild-type enzyme |
760261 |
| 2.7.7.60 | K213S |
effective turnover rate is ca. 0.4% of the rate of wild type, retains capacity to form product with significantly reduced efficiency |
393240 |
| 2.7.7.60 | K214S |
no activity in crude cell free extract |
661089 |
| 2.7.7.60 | K27A |
amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme |
393240 |
| 2.7.7.60 | K27A |
no activity in crude cell free extract |
661089 |
| 2.7.7.60 | K27S |
amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme |
393240 |
