EC Number |
Protein Variants |
Reference |
---|
2.7.8.11 | C148W |
polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence |
690824 |
2.7.8.11 | H114Q |
oligonucleotide-mediated site-directed mutagenesis, substitution of amino acid at position 114 from His (CAC) + Gln (CAA) results in a 200fold increase in Km of the enzyme for myo-inositol, making cells auxotrophic for myo-inositol |
645318 |
2.7.8.11 | Hy3 |
hybrid construct of 57 N-terminal amino acids from Saccharomyces cerevisiae PIS and 159 C-terminal amino acids from PfPIS, no in vitro activity, 60% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) |
677090 |
2.7.8.11 | Hy4 |
hybrid construct of 14 N-terminal amino acids from Saccharomyces cerevisiae PIS and 202 C-terminal amino acids from PfPIS, 37% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) |
677090 |
2.7.8.11 | K65C |
site-directed mutagenesis, cysteine installation for substituted cysteine analysis method, SCAM |
-, 737766 |
2.7.8.11 | more |
an addition of a KXKXX motif reduces the activity of Pis1 by a factor of 2 |
-, 737766 |
2.7.8.11 | more |
an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Homo sapiens cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 7 pmol/h/mg and 2 pmol/h/mg, respectively |
726887 |
2.7.8.11 | more |
an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Saccharomyces cerevisiae cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 107 pmol/h/mg and 7 pmol/h/mg, respectively |
726887 |
2.7.8.11 | more |
conditional double knockout mutant in the bloodstream form of Trypanosoma is not viable under nonpermissive conditions, which lead to a decrease in the amount of enzyme protein |
671861 |
2.7.8.11 | more |
gene successfully complements a Saccharomyces cerevisiae PIS1 deletion mutant |
677090 |