EC Number   |
Protein Variants |
Reference |
|---|
   4.2.1.119 | A348T |
site-directed mutagenesis, the mutation does not affect the enzyme |
730244 |
| 4.2.1.119 | A427V |
site-directed mutagenesis, the mutation does not affect the enzyme, the mutant shows a slight increase in activity |
730244 |
| 4.2.1.119 | A491T |
site-directed mutagenesis, the mutation does not affect the enzyme |
730244 |
| 4.2.1.119 | A606S |
site-directed mutagenesis, the mutant shows reduced activity |
730244 |
| 4.2.1.119 | D370A |
reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae |
652075 |
| 4.2.1.119 | D490A |
reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae |
652075 |
| 4.2.1.119 | D510A |
inactive mutant enzyme |
652075 |
| 4.2.1.119 | D510Y |
site-directed mutagenesis, inactive mutant, the mutation disrupts active site architecture |
730244 |
| 4.2.1.119 | D517A |
reduced specific acitivity of 2-enoyl-CoA hydratase 2 when expressed in Saccharomyces cerevisiae |
652075 |
| 4.2.1.119 | DELTA629-990 |
truncated version (lacking the carboxyl-terminal 271 amino acids). The truncated form contains only the D-3-hydroxyacyl-CoA dehydrogenase activity |
687487 |
