EC Number |
Protein Variants |
Reference |
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4.2.1.9 | more |
construction of a relaxed rel deletion Corynebacterium glutamicum DELTAilvA DELTApanB DELTArel ilvNM13 (pECKAilvBNC) strain with the ilvBNC operon present on the multicopy plasmid pECKA |
-, 715798 |
4.2.1.9 | more |
construction of a valine producer by genetic engineering, mutant strains are resistant against inhibition by branched-chain amino acids L-valine, L-isoleucine and L-leucine, A constructed weak promoter of ilvA or leuA, which is introduced into the Corynebacterium glutamicum chromosome via a gene-replacement technique, reduces the biosynthetic rate of isoleucine or leucine, which lowers the mutant growth rate and increases valine production. Overexpression of ilvD driven by the strong mutant promoter P-ilvDM7 resultsin an even higher level of valine production, engineered strains, overview |
699075 |
4.2.1.9 | more |
construction of strain BL21(DE3) DELTAilvD knockout mutant, functional complementation by Mycobacterium tuberculosis IlvD |
716148 |
4.2.1.9 | more |
engineering of wild-type Corynebacterium glutamicum for growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the constructed strains produce up to 188 mM 2-ketoisovalerate and show a product yield of about 0.47 mol per mol of glucose and a volumetric productivity of about 4.6 mM 2-ketoisovalerate per h in the overall production phase, overview |
713840 |
4.2.1.9 | more |
enzyme from iv-1 mutant strain 332 differs from the wild type enzyme with respect to heat-inactivation, pH-dependence, and kinetics. The altered structure of the mutant enzyme is the basic defect hindering its incorporation into mitochondria |
33967 |