1.13.12.6: Cypridina-luciferin 2-monooxygenase

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For detailed information about Cypridina-luciferin 2-monooxygenase, go to the full flat file.

Word Map on EC 1.13.12.6

1.13.12.6

bioluminescence

luminescence

hilgendorfii

luciferases

ostracod

firefly

analysis

gaussia

diagnostics

molecular biology

biotechnology

Reaction

Synonyms

Apogon luciferase, Apogon luciferase 1, CLase, CLuc, Cypridina luciferase, Cypridina luciferin 2-monooxygenase, Cypridina noctiluca luciferase, Cypridina-type luciferase, Fbp, FBP-IgG, luciferase (Cypridina luciferin), PGE2-luciferase, Vargula luciferase

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

1.13.12.6 Cypridina-luciferin 2-monooxygenase

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Results	in table
1	Activating Compound
5	AA Sequence
15	Application
1	CAS Registry Number
11	Cloned(Commentary)
4	General Information
2	General Stability
10	Inhibitors
10	KM Value [mM]
5	Localization
3	Metals/Ions
8	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
33	Organism
5	pH Optimum
1	pH Range
1	pI Value
3	Posttranslational Modification
16	Protein Variants
10	Purification (Commentary)
2	Reaction
3	Reaction Type
33	Reference
6	Source Tissue
1	Specific Activity [micromol/min/mg]
5	Storage Stability
31	Substrates and Products (Substrate)
6	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.13.12.6 - Cypridina-luciferin 2-monooxygenase

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(S)-Cypridina luciferin I + O2

oxidized S-Cypridina luciferin I + CO2 + hv

Vargula hilgendorfii

677182

1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine + O2

N-[3-(3-carbamimidamidopropyl)-5-(1H-indol-3-yl)-1,4-dihydropyrazin-2-yl]-2-methylbutanamide + CO2 + hn

Cypridina noctiluca

Q75R40

655065

Cypridina luciferin + O2

Cypridina noctiluca

fusion enzyme + 10 mM luciferin (dissolved in ethanol in 30 mM Tris-HCl (pH 7.6) with 10 mM EDTA) with ATP (250 mM), CoA (250 microM), and MgCl2 (5 mM) in 100 mM Tris-HCl, pH 7.8

696008

Cypridina luciferase fusion enzyme expressed in Escherichia coli is soluble but does not exhibit bioluminescence

Cypridina luciferin + O2

oxidized Cypridina luciferin + CO2 + hv

20 entries

DL-Cypridina luciferin + O2

oxidized DL-Cypridina luciferin + CO2 + hv

Vargula hilgendorfii

677182

luciferin + O2

oxidized luciferin + CO2 + hnu

Vargula hilgendorfii

439330

Vargula hilgendorfii luciferin + O2

oxidized Vargula hilgendorfii luciferin + CO2 + hv

Cypridina noctiluca

Q75R40

native or recombinant substrate protein

746376

additional information

Cypridina sp.

evaluation of a method of measuring activity of Cypridina luciferase under conditions which minimize complicating with a relatively simple photoelectric light integrator, detailed overview

744465