1.14.12.13: 2-halobenzoate 1,2-dioxygenase
This is an abbreviated version!
For detailed information about 2-halobenzoate 1,2-dioxygenase, go to the full flat file.
Word Map on EC 1.14.12.13
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1.14.12.13
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cepacia
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catechol
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chlorobenzoates
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burkholderia
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toluate
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chlorocatechols
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biphenyls
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rieske-type
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cupriavidus
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ortho-cleavage
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cydab
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regiospecificity
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necator
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polychlorinated
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quinol
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4-chlorocatechol
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benabc
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tn5-based
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monochlorobiphenyls
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2,4-dichlorobenzoate
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aroclor
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cis,cis-muconate
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co-oxidize
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minitransposon
- 1.14.12.13
- cepacia
- catechol
- chlorobenzoates
- burkholderia
- toluate
- chlorocatechols
- biphenyls
-
rieske-type
-
cupriavidus
-
ortho-cleavage
- cydab
-
regiospecificity
- necator
-
polychlorinated
- quinol
- 4-chlorocatechol
- benabc
-
tn5-based
-
monochlorobiphenyls
- 2,4-dichlorobenzoate
-
aroclor
- cis,cis-muconate
-
co-oxidize
-
minitransposon
Reaction
Synonyms
(chloro)benzoate dioxygenase, 2-chlorobenzoate 1,2-dioxygenase, 2-halobenzoate 1,2-dioxygenase, 2-halobenzoate dioxygenase, 2-halobenzoate dioxygenase system, BenA, CbdAB, cbdABC
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Engineering
Engineering on EC 1.14.12.13 - 2-halobenzoate 1,2-dioxygenase
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additional information
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mutants are made by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, mutants are unable to grow on 2-chlorobenzoate and catechol
additional information
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several mutants of Pseudomonas cepacia, putida and sp. are used
additional information
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disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8
additional information
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interposon mutagenesis, resulting strain TD2 fails to grow on benzoate or 2-chlorobenzoate showing that the cbd gene is involved in their degradation
additional information
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disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8
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