1.14.14.11: styrene monooxygenase
This is an abbreviated version!
For detailed information about styrene monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.11
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1.14.14.11
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epoxidation
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enantioselective
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two-component
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s-styrene
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synthesis
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phenylacetic
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solvent-tolerant
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enantiopure
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phenylacetaldehyde
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two-liquid
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opacus
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s-epoxides
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taiwanensis
- 1.14.14.11
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epoxidation
-
enantioselective
-
two-component
-
s-styrene
- synthesis
-
phenylacetic
-
solvent-tolerant
-
enantiopure
- phenylacetaldehyde
-
two-liquid
- opacus
-
s-epoxides
- taiwanensis
Reaction
Synonyms
FAD-specific styrene epoxidase, NSMOA, SMO, SmoA, StyA, StyA1, StyA1/StyA2B, StyA2B, StyAB
ECTree
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Engineering
Engineering on EC 1.14.14.11 - styrene monooxygenase
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L44A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
L45A
mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively
N46A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
R43A
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
L44A
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mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
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L45A
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mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively
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N46A
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mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
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R43A
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mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene
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T200V
mutant exhibits lower reactivity than the wild type, displayed impaired diastereoselectivity
additional information
the random mutation of styrene monooxygenase using error prone PCR generates a number of improved variants exhibiting the highest known rate of (S)-styrene epoxide and (1S-2R) indene epoxide production by a biocatalyst
additional information
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the random mutation of styrene monooxygenase using error prone PCR generates a number of improved variants exhibiting the highest known rate of (S)-styrene epoxide and (1S-2R) indene epoxide production by a biocatalyst
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additional information
construcution of fusion proteins that join the C-terminus of the epoxidase StyA to the N-terminus of FAD reductase StyB through a linker peptide and application in the synthesis of of a broad range of substituted indoles to indigoid chromophores. The fusion proteins are self-regulated and couple efficiently NADH oxidation to styrene epoxidation