1.14.19.19: sphingolipid 10-desaturase
This is an abbreviated version!
For detailed information about sphingolipid 10-desaturase, go to the full flat file.
Word Map on EC 1.14.19.19
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1.14.19.19
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photoproteins
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bioluminescence
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luminescence
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aequorin
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ca2+-regulated
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obelia
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longissima
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emit
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coelenteramide
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hydroid
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ef-hand
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ctenophore
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mnemiopsis
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ca2+-activated
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2-hydroperoxycoelenterazine
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ca2+-discharged
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calcium-activated
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coelenterates
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ca2+-binding
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leidyi
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jellyfish
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hydromedusan
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calcium-regulated
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medicine
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synthesis
- 1.14.19.19
-
photoproteins
-
bioluminescence
-
luminescence
- aequorin
-
ca2+-regulated
-
obelia
-
longissima
-
emit
- coelenteramide
-
hydroid
-
ef-hand
-
ctenophore
-
mnemiopsis
-
ca2+-activated
- 2-hydroperoxycoelenterazine
-
ca2+-discharged
-
calcium-activated
-
coelenterates
-
ca2+-binding
-
leidyi
-
jellyfish
-
hydromedusan
-
calcium-regulated
- medicine
- synthesis
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 + 2 H2O
Synonyms
DELTA 10(E)-SD, DELTA 10(E)-sphingolipid desaturase, DELTA10 desaturase, DesA, DesB, diene DELTA10-desaturase, sphingolipid 10,11-desaturase, sphingolipid DELTA10-desaturase
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Substrates Products
Substrates Products on EC 1.14.19.19 - sphingolipid 10-desaturase
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REACTION DIAGRAM
(4E,8E)-sphinga-4,8-dienine ceramide + ferrocytochrome b5 + O2 + H+
(4E,8E,10E)-sphinga-4,8,10-trienine ceramide + ferricytochrome b5 + H2O
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?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+
fusaruside + 2 ferricytochrome b5 + 2 H2O
(4E,8E)-sphinga-4,8-dienine ceramide + flavodoxin + O2 + H+
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?
fusaruside + 2 ferricytochrome b5 + 2 H2O
a (4E,8E)-sphinga-4,8-dienine ceramide
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?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+
fusaruside + 2 ferricytochrome b5 + 2 H2O
a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate
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-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+
fusaruside + 2 ferricytochrome b5 + 2 H2O
Fusarium graminearum CBS123657
a (4E,8E)-sphinga-4,8-dienine ceramide
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-
?
cerebroside B + 2 ferrocytochrome b5 + O2 + 2 H+
fusaruside + 2 ferricytochrome b5 + 2 H2O
Fusarium graminearum CBS123657
a (4E,8E)-sphinga-4,8-dienine ceramide, high substrate
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-
?
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LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis
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additional information
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Fusarium graminearum CBS123657
LC-MS screenings of sphingolipid-enriched fractions and in vitro enzymatic synthesis of fusaruside by recombinant DELTA 10(E)-SD. The enzyme shows no reactivity towards 2-methylbutene-bearing molecules including citral, isochromophilone VI, nerolidol, phytolorvitamin D1 and cerebroside B derived ceramide. The presence of the C9-methyl group is essential for the sphingolipid DELTA 10-desaturase activity, mutational analysis
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