1.14.19.22: acyl-lipid omega-6 desaturase (cytochrome b5) This is an abbreviated version! For detailed information about acyl-lipid omega-6 desaturase (cytochrome b5), go to the full flat file .
Reaction
an oleoyl-[glycerolipid] +
2 ferrocytochrome b5
+
O2 +
2 H+
=
a linoleoyl-[glycerolipid] +
2 ferricytochrome b5
+
2 H2O
Synonyms 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine DELTA12-desaturase, 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine DELTAl2-desaturase, 18:1-phosphatidylcholine desaturase, acyl-lipid DELTA12-desaturase, BrFAD2-1, CeFAT-2, CtFAD2-1, delta 12 fatty acid desaturase, delta-12 fatty acid desaturase 2, Delta-12 oleate desaturase, delta-twelve fatty acid desaturase 2, DELTA12 desaturase, DELTA12-desaturase, DELTA12-fatty acid desaturase, DELTA12-oleoyl-phosphatidylcholine desaturase, desaturase, oleate, DiFAD2, EC 1.3.1.35, endoplasmic reticulum-localized oleate desaturase, FAD2, FAD2-1, FAD2-1A, FAD2-1B, FAD2-2, FAD2-3, FAD2-4, FAD2A, FAD6, linoleate synthase, LKFAD15, microsomal oleate desaturase, microsomal oleic acid desaturase, microsomal omega-6 fatty acid desaturase, oleate desaturase, oleoyl phosphatidylcholine desaturase, oleoyl-CoA desaturase, oleoyl-PC desaturase, oleoyl-phosphatidylcholine desaturase, oleoylphosphatidylcholine desaturase, omega-6 desaturase, omega-6 fatty acid desaturase, omega-6-oleate desaturase, omega6-fatty acid desaturase, OsFAD2, plastidial oleate desaturase, seed-specific delta-12 fatty acid desaturase 2, SVE1, TauDELTA12des
ECTree
Cloned
Cloned on EC 1.14.19.22 - acyl-lipid omega-6 desaturase (cytochrome b5)
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CeFAT-2 is expressed under the control of an inducible Gal1 promoter in Saccharomyces cerevisiae
expresed in yeast cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Saccharomyces cerevisiae
expressed in Solanum tuberosum leaves as fusion protein with thermostable lichenase
-
expressed in yeast cells
-
expression in Saccahromyces cerevisae
flag-tagged enzyme is functionally expressed in yeast cells. To express the gene in thraustochytrids, a construct driven by the thraustochytrid-derived ubiquitin promoter is used
-
functional expression in Saccharomyces cerevisiae
gene desA, expression profile analysis, construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3, and expression in Escherichia coli strain XL1blue and Solanum tuberosum cells, the latter via Agrobacterium tumefaciens strain AGLO transfection. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of a recombinant hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction
-
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
recombinantly expressed in Saccharomyces cerevisiae and Pichia Pastoris
the pGEM-T Easy vector is used
CeFAT-2 is expressed under the control of an inducible Gal1 promoter in Saccharomyces cerevisiae
-
CeFAT-2 is expressed under the control of an inducible Gal1 promoter in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis