1.3.99.33: urocanate reductase
This is an abbreviated version!
For detailed information about urocanate reductase, go to the full flat file.
Reaction
Synonyms
SO_4620, urdA
ECTree
1.3.99.33 urocanate reductaseAdvanced search results
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results (Crystallization
Crystallization on EC 1.3.99.33 - urocanate reductase
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
two-domain construct of UrdA (UrdA') at four specific states: an ADP-bound structure without substrate, and three FAD-bound structures in complex with either the substrate urocanate or the product ImP, or without any ligand bound. For the urocanate and FAD-bound structure crystals: mixing of 30 mg/ml protein in 3.4 mM FAD and 3.0 mM urocanic acid with a reservoir solution consisting of 27% PEG 8000, 0.3 M (NH4)2SO4, 0.1 M HEPES, pH 7.0. Crystals in complex with imidazole propionate by mixing of 26 mg/ml protein in 20 mM HEPES, pH 7.0, 150 mM NaCl, 3.2 mM FAD, and 7 mM imidazole propionate with a reservoir solution containing 20% PEG 8000, 0.2 M NH4Cl, and 0.1 M HEPES, pH 7.0. Crystals for apo-FAD-bound structure by mixing of 0.001 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.0, 150 mM NaCl, and 3.2 mM FAD with 0.001 ml of reservoir solution containing 0.1 M Tris, pH 8.5, 2 M (NH4)2SO4, and 200 nl of 30% xylitol from the additive screen, X-ray diffraction structure determination and analysis at 1.1-2.56 A resolution
