1.5.1.38: FMN reductase (NADPH)
This is an abbreviated version!
For detailed information about FMN reductase (NADPH), go to the full flat file.
Reaction
Synonyms
(NADPH)-dependent flavin mononucleotide reductase, (NADPH)-dependent FMN reductase, BC_1619, EC 1.5.1.29, EC 1.6.8.1, flavin reductase P, FMN reductase, FRP, More, NAD(P)H:FMN reductase, NADPH specific FMN reductase, NADPH-flavin oxidoreductase, NADPH-FMN oxidoreductase, NADPH:FMN oxidoreductase, NADPH:FMN reductase, SsuE, ssueE, ycbP, ydgI
ECTree
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KM Value
KM Value on EC 1.5.1.38 - FMN reductase (NADPH)
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0.007
2-thioFMN
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pH 7.0, 23°C, enzyme derivative reconstituted from apoenzyme and 2-thioFMN
0.008
FMN
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pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation
0.003
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pH 7.0, 23°C, enzyme derivative reconstituted from apoenzyme and 2-thioFMN
0.02
NADPH
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pH 7.0, 23°C, single-enzyme spectrophotometric assay monitoring the NADPH oxidation
additional information
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the kinetic mechanism of FRP is changed to a sequential pattern with a Km(FMN) of 0.003 mM and a Km(NADPH) of 0.02 mM in a luciferase-coupled assay measuring light emission
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additional information
additional information
steady-state kinetic analysis of wild-type and mutant enzymes, kinetics of FMN binding, overview
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additional information
additional information
steady-state Michaelis-Menten kinetics, and rapid-reaction kinetic analyses of Y118A, DELTAY118 SsuE, and wild-type SsuE, as well as stopped-flow kinetics, overview
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