1.5.3.10: dimethylglycine oxidase
This is an abbreviated version!
For detailed information about dimethylglycine oxidase, go to the full flat file.
Word Map on EC 1.5.3.10
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1.5.3.10
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arthrobacter
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globiformis
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sarcosine
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formaldehyde
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tetrahydrofolate
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amine
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fad
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flavoprotein
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t-protein
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folate-binding
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folate
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betaine
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iminium
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multiwavelength
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half-reaction
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charge-transfer
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heterotetrameric
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folate-dependent
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folinic
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sulfite
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stopped-flow
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methylene
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aminomethyltransferase
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oxidases
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tetrahydrofolate-dependent
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l-proline
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tetrahydrofolate-binding
- 1.5.3.10
- arthrobacter
- globiformis
- sarcosine
- formaldehyde
- tetrahydrofolate
- amine
- fad
- flavoprotein
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t-protein
-
folate-binding
- folate
- betaine
-
iminium
-
multiwavelength
-
half-reaction
-
charge-transfer
-
heterotetrameric
-
folate-dependent
-
folinic
- sulfite
-
stopped-flow
-
methylene
- aminomethyltransferase
- oxidases
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tetrahydrofolate-dependent
- l-proline
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tetrahydrofolate-binding
Reaction
Synonyms
dimethylglycine dehydrogenase, dmg, DMGO, DMGO1, DMGO2, N,N-dimethylglycine:oxygen oxidoreductase (demethylating)
ECTree
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Substrates Products
Substrates Products on EC 1.5.3.10 - dimethylglycine oxidase
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REACTION DIAGRAM
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + O2
sarcosine + 5,10-methylenetetrahydrofolate + H2O2
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additional information
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bifunctional enzyme catalyzing the formation of 5,10-methylene tetrahydrofolate and the oxidation of N,N-dimethylglycine, folate binding and active site, a large channel connects both active sites, the channeling is kinetically controlled by terahydrofolate binding, overview
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sarcosine + formaldehyde + H2O2
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
involved in tetrahydrofolate-dependent assimilation of methyl groups
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
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the oxidation of dimethylglycine is coupled to the synthesis of 5,10-methylenetetrahydrofolate through an unusual substrate channeling mechanism. Uncoupling of the active sites can be achieved by mutagenesis or deletion of the 5,10-methylenetetrahydrofolate synthase site and this leads to accumulation of intracellular formaldehyde. Channeling occurs by nonbiased diffusion of the labile intermediate through a large solvent cavity connecting both active sites
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
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In the absence of tetrahydrofolate, formaldehyde is the second product of catalysis, formed by hydrolysis of a labile iminium intermediate. In the cell, toxic formaldehyde is not produced and these enzymes catalyse the formation of 5,10-methylene-THF from THF and the labile iminium intermediate.
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
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N,N-dimethylglycine + H2O + O2
sarcosine + formaldehyde + H2O2
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