2.1.1.174: 23S rRNA (guanine1835-N2)-methyltransferase
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For detailed information about 23S rRNA (guanine1835-N2)-methyltransferase, go to the full flat file.
Reaction
Synonyms
23S rRNA methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G1835, N2-methyltransferase RlmG, ribosomal RNA large subunit methyltransferase G, RlmG, ygjO
ECTree
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Cloned
Cloned on EC 2.1.1.174 - 23S rRNA (guanine1835-N2)-methyltransferase
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expression of C-terminally His6-tagged full-length enzyme, expression of the C-terminal domain and the N-terminal domain as SUMO fusion proteins, the C-terminal domain is unstable after detagging through ubiquitin-like-specific protease 1 cleavage, while the N-terminal domain is stable
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli