2.1.1.205: tRNA (cytidine32/guanosine34-2'-O)-methyltransferase

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For detailed information about tRNA (cytidine32/guanosine34-2'-O)-methyltransferase, go to the full flat file.

Reaction

Synonyms

trm7, Trm7p

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.205 tRNA (cytidine32/guanosine34-2'-O)-methyltransferase

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Results	in table
2474	AA Sequence
1	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
7	General Information
3	Localization
1	Metals/Ions
7	Natural Substrates/ Products (Substrates)
3	Organism
1	Pathway
4	PDB ID
1	pH Optimum
1	Protein Variants
1	Purification (Commentary)
1	Reaction
3	Reference
10	Substrates and Products (Substrate)
2	Subunits
5	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.1.1.205 - tRNA (cytidine32/guanosine34-2'-O)-methyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme complexes Trm7-Trm734 and Trm7-Trm734-SAM, hanging drop vapor diffusion technique, mixing of 3 mg/ml protein solution with reservoir solution containing 0.1 M HEPES-NaOH, pH 7.5, 2 M (NH4)2SO4, 2% PEG 400, 2 mM phenol, and 3% ethylene-glycol, and equilibration against 0.2m ml reservoir solution, soaking of the crystals in in the mother liquor containing 1 mM SAM for complex 2, 20°C, 5 days, X-ray diffraction structure determination and analysis at 2.32-3.20 A resolution

Saccharomyces cerevisiae

P38238

757855