2.1.1.224: 23S rRNA (adenine2503-C8)-methyltransferase

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For detailed information about 23S rRNA (adenine2503-C8)-methyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

antibiotic resistance protein, Ba Cfr, C8 adenine RNA methylase, Cd Cfr, Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase, cfr(C), Cfr(E), cfr-like, Cfr-like protein, ClbA, ClbB, ClbC, EC 2.1.1.194, Ef Cfr, Pl Cfr, radical AdoMet rRNA methyltransferase, radical S-adenosylmethionine enzyme, radical S-adenosylmethionine methylase, radical-S-adenosyl-L-methionine enzyme, radical-SAM enzyme, radical-SAM rRNA methyltransferase, RS methylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.224 23S rRNA (adenine2503-C8)-methyltransferase

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Results	in table
1	Activating Compound
245	AA Sequence
1	CAS Registry Number
21	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
2	Expression
66	General Information
5	KM Value [mM]
10	Metals/Ions
3	Molecular Weight [Da]
35	Natural Substrates/ Products (Substrates)
65	Organism
1	Pathway
7	pH Optimum
28	Protein Variants
3	Purification (Commentary)
6	Reaction
41	Reference
2	Source Tissue
60	Substrates and Products (Substrate)
4	Subunits
82	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]

Systematic Name

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Systematic Name on EC 2.1.1.224 - 23S rRNA (adenine2503-C8)-methyltransferase

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SYSTEMATIC NAME

IUBMB Comments

S-adenosyl-L-methionine:23S rRNA (adenine2503-C8)-methyltransferase

This enzyme is a member of the 'AdoMet radical' (radical SAM) family. S-Adenosyl-L-methionine acts as both a radical generator and as the source of the appended methyl group. It contains an [4Fe-4S] cluster [3,6,7]. Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics [1]. The enzyme methylates adenosine at position 2503 of 23S rRNA by a radical mechanism, transferring a CH2 group from S-adenosyl-L-methionine while retaining the hydrogen at the C-8 position of the adenine. Cfr first transfers an CH2 group to a conserved cysteine (Cys338 in Staphylococcus aureus) [7], the generated radical from a second S-adenosyl-L-methionine then attacks the methyl group, exctracting a hydrogen. The formed radical forms a covalent intermediate with the adenine group of the tRNA [8]. The enzyme will also methylate 2-methyladenine produced by the action of EC 2.1.1.192 [23S rRNA (adenine2503-C2)-methyltransferase].