2.1.2.B2: cytidylate 5-hydroxymethyltransferase

This is an abbreviated version!
For detailed information about cytidylate 5-hydroxymethyltransferase, go to the full flat file.

Reaction

Synonyms

CMP hydroxymethylase, cytidylate hydroxymethylase, MilA, thymidylate synthase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.B2 cytidylate 5-hydroxymethyltransferase

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Results	in table
1	AA Sequence
2	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
10	General Information
2	kcat/KM [mM/s]
2	KM Value [mM]
6	Natural Substrates/ Products (Substrates)
7	Organism
1	pH Optimum
3	Protein Variants
2	Purification (Commentary)
1	Reaction
4	Reference
15	Substrates and Products (Substrate)
2	Subunits
10	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.1.2.B2 - cytidylate 5-hydroxymethyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

5,10-methylenetetrahydrofolate + H2O + CMP

tetrahydrofolate + 5-hydroxymethyl-CMP

4 entries

5,10-methylenetetrahydrofolate + H2O + cytidylate

tetrahydrofolate + 5-hydroxymethylcytidylate

3 entries

5,10-methylenetetrahydrofolate + H2O + dCMP

tetrahydrofolate + 5-hydroxymethyl-dCMP

2 entries

5,10-methylenetetrahydrofolate + H2O + deoxycytidylate

tetrahydrofolate + 5-hydroxymethyldeoxycytidylate

Streptomyces rimofaciens

B4Y380

-

758390

-

-

?

additional information

?

-

6 entries

5,10-methylenetetrahydrofolate + H2O + CMP

tetrahydrofolate + 5-hydroxymethyl-CMP

Streptomyces rimofaciens

B4Y380

-

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + CMP

tetrahydrofolate + 5-hydroxymethyl-CMP

Streptomyces rimofaciens

B4Y380

CMP is the strongly preferred substrate over dCMP

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + CMP

tetrahydrofolate + 5-hydroxymethyl-CMP

Streptomyces rimofaciens ZJU5119

-

-

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + CMP

tetrahydrofolate + 5-hydroxymethyl-CMP

Streptomyces rimofaciens ZJU5119

-

CMP is the strongly preferred substrate over dCMP

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + cytidylate

tetrahydrofolate + 5-hydroxymethylcytidylate

Streptomyces rimofaciens

-

-

735967, 758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + cytidylate

tetrahydrofolate + 5-hydroxymethylcytidylate

Streptomyces rimofaciens

B4Y380

-

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + cytidylate

tetrahydrofolate + 5-hydroxymethylcytidylate

Streptomyces rimofaciens ZJU5119

-

-

735967

-

-

?

5,10-methylenetetrahydrofolate + H2O + dCMP

tetrahydrofolate + 5-hydroxymethyl-dCMP

Streptomyces rimofaciens

B4Y380

-

758390

-

-

?

5,10-methylenetetrahydrofolate + H2O + dCMP

tetrahydrofolate + 5-hydroxymethyl-dCMP

Streptomyces rimofaciens ZJU5119

-

-

758390

-

-

?

additional information

?

-

Streptomyces rimofaciens

-

5-hydroxymethylcytosine is present in a nucleoside antibiotic mildiomycin and its formation is governed by a cytidylate hydroxymethylase MilA

735967

-

-

?

additional information

?

-

Streptomyces rimofaciens

-

MilA uses solvent water as the hydroxyl group donor like cytidylate hydroxymethylase. Thr102 of MilA is a potential critical amino acid anchoring one molecule of water for hydroxylation. Thr102 of MilA is predicted as potential critical amino acid anchoring one molecule of water for hydroxylation

735967

-

-

?

additional information

?

-

Streptomyces rimofaciens

-

MilA catalyzes the conversion of CMP into 5-hydroxymethyl-CMP with an efficiency (kcat/KM) of 5fold faster than for deoxycytidylate

758390

-

-

-

additional information

?

-

Streptomyces rimofaciens

B4Y380

MilA catalyzes the conversion of CMP into 5-hydroxymethyl-CMP with an efficiency (kcat/KM) of 5fold faster than for deoxycytidylate

758390

-

-

-

additional information

?

-

Streptomyces rimofaciens ZJU5119

-

5-hydroxymethylcytosine is present in a nucleoside antibiotic mildiomycin and its formation is governed by a cytidylate hydroxymethylase MilA

735967

-

-

?

additional information

?

-

Streptomyces rimofaciens ZJU5119

-

MilA uses solvent water as the hydroxyl group donor like cytidylate hydroxymethylase. Thr102 of MilA is a potential critical amino acid anchoring one molecule of water for hydroxylation. Thr102 of MilA is predicted as potential critical amino acid anchoring one molecule of water for hydroxylation

735967

-

-

?