2.4.1.239: flavonol-3-O-glucoside glucosyltransferase

This is an abbreviated version!
For detailed information about flavonol-3-O-glucoside glucosyltransferase, go to the full flat file.

Reaction

Synonyms

flavonol 3-O-glucoside/galactoside (1->2) glucosyltransferase, GmF3G2''Gt, UDP-glucose:glucosyltransferase, UGT79B30

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.239 flavonol-3-O-glucoside glucosyltransferase

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Results	in table
2	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
1	Cofactor
2	General Information
2	Inhibitors
2	KM Value [mM]
1	Metals/Ions
2	Natural Substrates/ Products (Substrates)
3	Organism
6	Pathway
2	pH Optimum
1	Protein Variants
1	Purification (Commentary)
2	Reaction
1	Reaction Type
3	Reference
4	Source Tissue
1	Specific Activity [micromol/min/mg]
2	Storage Stability
15	Substrates and Products (Substrate)
4	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 2.4.1.239 - flavonol-3-O-glucoside glucosyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

TDP-glucose + a flavonol 3-O-beta-D-glucoside

TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

3fold lower rate than with UDP-glucose as glucosyl donor

654323

-

-

?

UDP-glucose + a flavonol 3-O-beta-D-glucoside

UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

highly specific enzyme, specific for flavonol-3-O-monoglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor

654323

-

-

?

UDP-glucose + a flavonol 3-O-beta-D-glucoside

UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside

Glycine max

I1KEV6

-

735863

-

-

?

UDP-glucose + kaempferol 3-O-beta-D-glucoside

UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

2 entries

UDP-glucose + kaempferol 3-O-glucoside

UDP + kaempferol 3-O-sophoroside

Glycine max

I1KEV6

activity of the enzyme from cultivar Harosoy

735863

-

-

?

UDP-glucose + myricetin 3-O-beta-D-glucoside

UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

50% of the activity with kaempferol 3-O-beta-D-glucoside

654323

-

-

?

UDP-glucose + pelargonidin 3-O-beta-D-glucoside

UDP + pelargonidin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

2% of the activity with kaempferol 3-O-beta-D-glucoside

654323

-

-

?

UDP-glucose + quercetin 3-O-beta-D-glucoside

UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

2 entries

additional information

?

-

6 entries

UDP-glucose + kaempferol 3-O-beta-D-glucoside

UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

best substrate

654323

-

-

?

UDP-glucose + kaempferol 3-O-beta-D-glucoside

UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

glucosylates kaempferol monoglucoside and quercetin monoglucoside equally

657425

-

-

?

UDP-glucose + quercetin 3-O-beta-D-glucoside

UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

62% of the activity with kaempferol 3-O-beta-D-glucoside

654323

-

-

?

UDP-glucose + quercetin 3-O-beta-D-glucoside

UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside

Pisum sativum

-

glucosylates kaempferol monoglucoside and quercetin monoglucoside equally

657425

-

-

?

additional information

?

-

Glycine max

I1KEV6

the enzyme of cultivar Nezumisaya shows a broad activity for kaempferol/quercetin 3-O-glucoside/galactoside derivatives but does not glucosylate kaempferol 3-O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)-glucoside] and 3-O-rhamnosyl-(1->4)-[glucosyl-(1->6)-glucoside]

735863

-

-

?

additional information

?

-

Glycine max

-

the enzyme of cultivar Nezumisaya shows a broad activity for kaempferol/quercetin 3-O-glucoside/galactoside derivatives but does not glucosylate kaempferol 3-O-rhamnosyl-(1->4)-[rhamnosyl-(1->6)-glucoside] and 3-O-rhamnosyl-(1->4)-[glucosyl-(1->6)-glucoside]

735863

-

-

?

additional information

?

-

Pisum sativum

-

involved in the biosynthesis of flavonol triglucosides

657425

-

-

?

additional information

?

-

Pisum sativum

-

one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides

654323

-

-

?

additional information

?

-

Pisum sativum

-

glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities

657425

-

-

?

additional information

?

-

Pisum sativum

-

no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol diglucoside, quercetin-3-rutinoside, quercetin-3-rhamnoside

654323

-

-

?