2.4.1.85: cyanohydrin beta-glucosyltransferase

This is an abbreviated version!
For detailed information about cyanohydrin beta-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

cyanohydrin glucosyltransferase, cyanohydrin glycosyltransferase, glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile, mandelonitrile glucosyltransferase, sbHMNGT, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase, UDP-glucosyltransferase, UGT85B1, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin beta-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.85 cyanohydrin beta-glucosyltransferase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
3	Activating Compound
43	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
6	General Information
2	General Stability
6	Inhibitors
12	KM Value [mM]
8	Localization
1	Metals/Ions
1	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
12	Organism
4	Pathway
2	PDB ID
2	pH Optimum
6	Protein Variants
3	Purification (Commentary)
1	Reaction
1	Reaction Type
12	Reference
16	Source Tissue
2	Specific Activity [micromol/min/mg]
2	Storage Stability
46	Substrates and Products (Substrate)
2	Subunits
17	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
10	Turnover Number [1/s]

Engineering

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Engineering on EC 2.4.1.85 - cyanohydrin beta-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E410A

Sorghum bicolor

Q9SBL1

glucosylation of mandelonitrile is 225fold reduced compared to wild-type enzyme

660260

R201A

Sorghum bicolor

Q9SBL1

glucosylation of mandelonitrile is 20fold reduced compared to wild-type enzyme

660260

S391A

Sorghum bicolor

Q9SBL1

glucosylation of mandelonitrile is 185fold reduced compared to wild-type enzyme

660260

additional information

6 entries

additional information

Sorghum bicolor

Q9SBL1

transgenic Arabidopsis thaliana plants that produce dhurrin are obtained by co-expression of CYP79A1/CYP71E1-CFP (cyan fluorescent protein)/UGT85B1-YFP (yellow fluorescent protein) and of CYP79A1/CYP71E1/UGT85B1-YFP (yellow fluorescent protein)

694605

additional information

Sorghum bicolor

-

transgenic Arabidopsis thaliana plants that produce dhurrin are obtained by co-expression of CYP79A1/CYP71E1-CFP (cyan fluorescent protein)/UGT85B1-YFP (yellow fluorescent protein) and of CYP79A1/CYP71E1/UGT85B1-YFP (yellow fluorescent protein)

694605

additional information

Sorghum bicolor

Q9SBL1

construction of a knockout mutant by use of targeted induced local lesions in genomes (TILLING) to identify a line with a mutation resulting in a premature stop codon in the N-terminal region of UGT85B1. Plants homozygous for this mutation do not produce dhurrin and are designated tcd2 (totally cyanide deficient 2) mutants. Phenotype, detailed overview

736976

additional information

Sorghum bicolor

-

construction of a knockout mutant by use of targeted induced local lesions in genomes (TILLING) to identify a line with a mutation resulting in a premature stop codon in the N-terminal region of UGT85B1. Plants homozygous for this mutation do not produce dhurrin and are designated tcd2 (totally cyanide deficient 2) mutants. Phenotype, detailed overview

736976

additional information

Sorghum bicolor

Q9SBL1

engineering for introduction of the dhurrin biosynthesis pathway, starting with L-tyrosine and comprising the enzymes CYP79A1, CYP71E1, and UGT85B1 into Nicotiana tabacum. Integration of genes CYP79A1, CYP71E1, and UGT85B1 into a neutral site of the Nicotiana tabacum chloroplast genome and functional expression, overview. The enzymes convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounted to 0.1-0.2% of leaf dry weight compared to 6% in the origin Sorghum bicolor

736574

additional information

Sorghum bicolor

-

engineering for introduction of the dhurrin biosynthesis pathway, starting with L-tyrosine and comprising the enzymes CYP79A1, CYP71E1, and UGT85B1 into Nicotiana tabacum. Integration of genes CYP79A1, CYP71E1, and UGT85B1 into a neutral site of the Nicotiana tabacum chloroplast genome and functional expression, overview. The enzymes convert endogenous tyrosine into dhurrin using electrons derived directly from the photosynthetic electron transport chain, without the need for the presence of an NADPH-dependent P450 oxidoreductase. The dhurrin produced in the engineered plants amounted to 0.1-0.2% of leaf dry weight compared to 6% in the origin Sorghum bicolor

736574