2.6.1.30: pyridoxamine-pyruvate transaminase
This is an abbreviated version!
For detailed information about pyridoxamine-pyruvate transaminase, go to the full flat file.
Word Map on EC 2.6.1.30
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2.6.1.30
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pyridoxal
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mesorhizobium
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loti
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5'-phosphate
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transamination
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5'-deoxypyridoxal
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l-alanine
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pyridoxine
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nabh4
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4-oxidase
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analysis
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synthesis
- 2.6.1.30
- pyridoxal
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mesorhizobium
- loti
- 5'-phosphate
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transamination
- 5'-deoxypyridoxal
- l-alanine
- pyridoxine
- nabh4
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4-oxidase
- analysis
- synthesis
Reaction
Synonyms
aminotransferase, pyridoxamine-pyruvate, PM-pyruvate transaminase, PPAT, pyridoxamine-pyruvate aminotransferase, pyridoxamine-pyruvic transaminase
ECTree
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Engineering
Engineering on EC 2.6.1.30 - pyridoxamine-pyruvate transaminase
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C198A
E68A
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
E68G
low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
K197L
V70K
active site residue, increase in reactivity toward 2-oxoglutarate
E68A
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low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
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E68G
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low pyridoxamine 5'-phosphate:pyruvate aminotransferase activity, decrease in activity towards pyridoxamine
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additional information
enzyme shows the same specific activity as that of the wild type enzyme
pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
additional information
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pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
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additional information
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pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
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