2.7.1.165: glycerate 2-kinase

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For detailed information about glycerate 2-kinase, go to the full flat file.

Reaction

Synonyms

2-PGA forming glycerate kinase, class II GK, class II glycerate kinase, D-glycerate 2-kinase, D-glycerate kinase, D-glycerate-2-kinase, EcoGK1, garK, GCK, glxK, glycerate kinase, glycerate kinase (2-phosphoglycerate forming), Glyctk, GLYK, More, slr1840, Sso-GK, SSO0666, SynGK, Ta0453, TM1585, TM_1585

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.165 glycerate 2-kinase

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Results	in table
3	Activating Compound
1331	AA Sequence
6	Application
12	Cloned(Commentary)
5	Cofactor
1	Crystallization (Commentary)
5	General Information
3	General Stability
33	Inhibitors
1	kcat/KM [mM/s]
4	Ki Value [mM]
25	KM Value [mM]
51	Metals/Ions
30	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
73	Organism
22	Pathway
8	pH Optimum
2	pH Range
3	pH Stability
1	pI Value
4	Protein Variants
15	Purification (Commentary)
1	Reaction
29	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
7	Storage Stability
85	Substrates and Products (Substrate)
21	Subunits
48	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
8	Temperature Range [°C]
15	Temperature Stability [°C]
14	Turnover Number [1/s]

Engineering

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Engineering on EC 2.7.1.165 - glycerate 2-kinase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

F483S

Homo sapiens

-

the mutant shows loss of enzyme activity. The mutation leads to D-glyceric acidemia

722379

F493C

Homo sapiens

-

the mutant shows loss of enzyme activity. The mutation leads to D-glyceric acidemia

722379

L510C

Homo sapiens

-

the mutant shows loss of enzyme activity. The mutation leads to D-glyceric acidemia

722379

additional information

Escherichia coli

P23524

development of a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables D-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways leads to the development of a DELTAgarKDELTAhyiDELTAglxKDELTAuxaC mutant strain that produces 4.8 g/l of D-glyceric acid from D-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produces similar yields and demonstrates that galactose or glycerol serve as possible carbon co-feeds for industrial production, method overview

759581