Information on EC 1.1.1.126 - 2-dehydro-3-deoxy-D-gluconate 6-dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY hide
1.1.1.126
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RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxy-D-gluconate 6-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-3-deoxy-D-gluconate + NADP+ = (4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH + H+
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alginate degradation
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-3-deoxy-D-gluconate:NADP+ 6-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-55-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
alginate-adapted
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
-
irreversible at pH 7.0, 1% reversibility at pH 10.0
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate + NADPH
2-dehydro-3-deoxy-D-gluconate + NADP+
show the reaction diagram
-
irreversible at pH 7.0, 1% reversibility at pH 10.0
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
7.2% of the rate compared to NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-hydroxymercuribenzoate
-
26% inhibition at 0.1 mM
Tris-HCl
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67% inhibition at pH 7.5, 50 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.36
(4S,5S)-4,5-dihydroxy-2,6-dioxohexanoate
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-
0.032
NADPH
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
inducible enzyme synthesis by growth in alginate-containing medium
Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in the absence of NADP+
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-3°C, 1 mM NADP+, 90% retaining activity after 3 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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