Information on EC 1.1.1.278 - 3beta-hydroxy-5alpha-steroid dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.278
-
RECOMMENDED NAME
GeneOntology No.
3beta-hydroxy-5alpha-steroid dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3beta-hydroxy-5alpha-pregnane-20-one + NADP+ = 5alpha-pregnan-3,20-dione + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cardenolide biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxy-5alpha-steroid:NADP+ 3-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9015-81-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain VIII
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
male Long-Evans, Turku strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3beta-hydroxy-16alpha,17alpha-epoxypregn-5-en-20-one + NADP+
?
show the reaction diagram
-
is metabolized through the putative 3beta-HSD/isomerase pathway
-
-
?
5alpha-pregnan-3,20-dione + NAD(P)H
3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+
show the reaction diagram
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
show the reaction diagram
dehydroepiandrosterone + NADP+
?
show the reaction diagram
pregnenolone + NADP+
?
show the reaction diagram
-
is metabolized through the putative 3beta-HSD/isomerase pathway
-
-
?
additional information
?
-
-
progesterone is no substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5alpha-pregnan-3,20-dione + NADPH
3beta-hydroxy-5alpha-pregnane-20-one + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activates at 1 mM, inhibitory at 10 mM
2-mercaptoethanol/cysteine
-
-
-
5alpha-pregnan-3,20-dione
-
-
ascorbate
-
-
NADPH
-
inhibitory at concentrations above 1 mM
progesterone
-
-
additional information
-
presence of C-7 allylic hydroxylation, in either epimeric form, inhibits 3beta-HSD/isomerase activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
-
1 mM, 1.75fold increase in activity
additional information
-
3beta-HSD/isomerase activity only occurs following lactonization of the steroidal ring-D
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0185 - 0.02
5alpha-pregnan-3,20-dione
-
pH 8.0, 25C
0.05 - 0.12
NADPH
-
pH 8.0, 25C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0095 - 0.0125
5alpha-pregnan-3,20-dione
0.035 - 0.066
progesterone
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 35
-
half-maximal activity at 5C and 35C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity below detection limit of 0.1 fmol/mg protein/min
Manually annotated by BRENDA team
-
anterior, low activity
Manually annotated by BRENDA team
-
pubic
Manually annotated by BRENDA team
-
light and dark-grown
Manually annotated by BRENDA team
additional information
-
proembryonic mass
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE