Information on EC 1.2.1.21 - glycolaldehyde dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.21
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RECOMMENDED NAME
GeneOntology No.
glycolaldehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycolaldehyde + NAD+ + H2O = glycolate + NADH + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-arabinose degradation I
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ethylene glycol degradation
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degradation of pentoses
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Glyoxylate and dicarboxylate metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
glycolaldehyde:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-89-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Alcaligenes viscolactis
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Manually annotated by BRENDA team
strain IFO 3007
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Manually annotated by BRENDA team
strain B WG1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Protaminobacter alboflavus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
betaine + NADH
betaine aldehyde + NAD+ + H2O
show the reaction diagram
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isozyme A 139% of activity with glycolate, isozyme B, 76%
glucono-delta-lactone is not used as substrate
?
D,L-glyceraldehyde + NAD+ + H2O
2,3-dihydroxypropanoic acid + NADH
show the reaction diagram
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-
-
?
formate + NADH
methanal + NAD+
show the reaction diagram
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isozyme A, 163% of activity with glycolate
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?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
show the reaction diagram
glyoxylate + NADH
glyoxal + NAD+
show the reaction diagram
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isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150%
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?
hydroxypyruvate + NAD+ + H2O
?
show the reaction diagram
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?
malonate + NADH
malonic semialdehyde + NAD+ + H2O
show the reaction diagram
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isozyme A, 83% of activity with glycolate
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?
oxalate + NADH
glyoxylate + NAD+
show the reaction diagram
propionaldehyde + NAD+ + H2O
propionate + NADH
show the reaction diagram
succinate + NADH
succinic semialdehyde + NAD+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
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1 mM, isozyme A, 26% inhibition
As3+
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1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Ca2+
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1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Co2+
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1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
glycolaldehyde
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substrate inhibition
glycolate
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substrate inhibition
HgCl2
KCN
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isozymes A and C
Mg2+
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1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Mn2+
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1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition
monoiodoacetate
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1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition
MoO22-
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1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
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p-chloromercuribenzoate
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0.1 mM, 36% inhibition
pyridoxal
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0.01 mM, 40% inhibition of total enzyme activity in cell extracts
pyridoxamine
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approx. 0.1 mM, 22% inhibition of isozyme A
pyridoxamine phosphate
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approx. 0.1 mM, 23% inhibition of isozyme A
pyridoxine
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approx. 0.1 mM, 33% inhibition of isozyme C, 0.39 mM, 50% inhibition
pyridoxine phosphate
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approx. 0.1 mM, 23% inhibition of isozyme C
Zn2+
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1 mM, isozyme A, 30% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal
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approx. 0.1 mM, 1.4fold increase in activity of isozyme A
pyridoxal 5'-phosphate
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approx. 0.1 mM, 1.5fold increase in activity of isozyme B
pyridoxine
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0.1 mM, 1.5fold increase in activity of isozyme B
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038
acetaldehyde
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0.00023
benzaldehyde
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0.027 - 27
glycolaldehyde
0.000000059 - 11
glycolate
0.067 - 0.15
NADP+
0.025 - 0.15
NADPH
0.023
propionaldehyde
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021 - 8.3
glycolaldehyde
0.00085 - 0.13
glycolate
0.33
pyridoxine
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inhibition of isozyme C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
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enzyme activity in crude extracts of temperature sensitive strain JA-104 at 42C
0.042
Alcaligenes viscolactis
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glycolate reduction in cell extracts
0.1
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glycolate reduction in cell extracts
0.105
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glycolate reduction in cell extracts
0.11
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glycolate reduction in cell extracts
0.13
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enzyme activity in crude extracts of strain 1 grown on casein hydrosylate
0.136
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glycolate reduction in cell extracts
0.145
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enzyme activity in crude extracts of wild-type strain G1
0.165
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enzyme activity in crude extracts of wild-type strain G3
0.187
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glycolate reduction in cell extracts
0.21
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enzyme activity in crude extracts of strain 1 grown on fucose
0.23
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glycolate reduction in cell extracts
0.262
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glycolaldehyde oxidation in cell extracts
0.267
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glycolaldehyde oxidation in cell extracts
0.3
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glycoldehyde oxidation in cell extracts
0.325
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glycolaldehyde oxidation in cell extracts
0.34
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enzyme activity in crude extracts of strain JA-102 grown on ethylene glycol
0.362
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glycolate reduction in cell extracts
0.56
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enzyme activity in crude extracts of ethylene glycol positive mutant strain EG3
0.825
Protaminobacter alboflavus
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glycolate reduction in cell extracts
0.927
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glycolate reduction in cell extracts
1.079
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glycolaldehyde oxidation in cell extracts
1.549
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strain IFO 3009, glycolate reduction in cell extracts
1.65
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glycolaldehyde oxidation in cell extracts
2.7
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isozyme B
2.75
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isozyme C
3.66
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isozyme A
6.38
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isozyme A from pyridoxine auxotroph strain WG3
10.39
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glycolaldehyde oxidation in cell extracts
10.5
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strain 3
30.5
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strain JA-102
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
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glycolate reduction, isozyme A, B
5.4
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isozyme A, glycolaldehyde oxidation
6
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glycolate reduction, isozyme C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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isozyme A
30
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isozyme A from strain WG3
45
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isozymes B and C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
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isozyme B, gel filtration
225000
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isozyme A, gel filtration
345000
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isozyme C, gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes
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unstable enzyme, not stabilized by PMSF
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
isozymes A and B
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partial: isozymes A, B and C
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protein identical to lactaldehyde dehydrogenase
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Show AA Sequence (208 entries)
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