Information on EC 1.2.1.61 - 4-hydroxymuconic-semialdehyde dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.61
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RECOMMENDED NAME
GeneOntology No.
4-hydroxymuconic-semialdehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-hydroxymuconic semialdehyde + NAD+ + H2O = maleylacetate + NADH + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxyacetophenone degradation
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4-nitrophenol degradation I
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Chlorocyclohexane and chlorobenzene degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
4-hydroxymuconic-semialdehyde:NAD+ oxidoreductase
Involved in the 4-nitrophenol degradation pathway.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
strain ACB
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Manually annotated by BRENDA team
strain ACB
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Manually annotated by BRENDA team
isolated from methyl parathion polluted activated sludge
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Manually annotated by BRENDA team
isolated from methyl parathion polluted activated sludge
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxymuconic semialdehyde + NAD+ + H2O
maleylacetate + NADH
show the reaction diagram
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conversion of hydroquinone to beta-ketoadipic acid via 4-hydroxymuconic semialdehyde and maleyl acetic acid
-
?
4-hydroxymuconic semialdehyde + NAD+ + H2O
maleylacetate + NADH + 2 H+
show the reaction diagram
4-hydroxymuconic semialdehyde + NAD+ + H2O
maleylacetate + NADH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxymuconic semialdehyde + NAD+ + H2O
maleylacetate + NADH
show the reaction diagram
-
conversion of hydroquinone to beta-ketoadipic acid via 4-hydroxymuconic semialdehyde and maleyl acetic acid
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
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5 mM chloride salt, 123.3% activity compared to untreated control
Cd2+
-
5 mM sulfate salt, 58.9% activity compared to untreated control
Co2+
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5 mM chloride salt, 147.1% activity compared to untreated control
Cu2+
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5 mM chloride salt, 110.2% activity compared to untreated control
Fe2+
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5 mM chloride salt, 39.5% activity compared to untreated control
Fe3+
-
5 mM chloride salt, 187.4% activity compared to untreated control
K+
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5 mM chloride salt, 95.7% activity compared to untreated control
Mg2+
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5 mM chloride salt, 138.1% activity compared to untreated control
Mn2+
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5 mM chloride salt, 186.1% activity compared to untreated control
Na+
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5 mM chloride salt, 88.2% activity compared to untreated control
Ni2+
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5 mM chloride salt, 183.9% activity compared to untreated control
Zn2+
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5 mM chloride salt, 145.9% activity compared to untreated control
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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5 mM, 83.2% activity compared to untreated control
SDS
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5 mM, 81.6% activity compared to untreated control
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0006
uninduced cells, pH 7.0, temperature not specified in the publication
0.0025
hydroquinone-induced cells, pH 7.0, temperature not specified in the publication
0.0031
nonylphenol-induced cells, pH 7.0, temperature not specified in the publication
0.44
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purified recombinant His6-PdcG enzyme, pH 8.0 at 50°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 70
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
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recombinant His6-PdcG by SDS-PAGE
53500
calculated from sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
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18% activity after 30 min at pH 3.0, and 75% activity after 30 min at pH 10.0
719179
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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purified enzyme retains 65% activity after 20 min at 60°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His6-PdcG by Ni2+-NTA affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HqdC expression in Escherichia coli BL21-DE3 cells
PdcG with His6 tag inserted into expression vectors pET30a and expressed in Escherichia coli BL21 (DE3) cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
by 0.5 mM hydroquinone and by 0.5 mM nonylphenol
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information