Information on EC 1.4.1.15 - lysine dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.4.1.15
-
RECOMMENDED NAME
GeneOntology No.
lysine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-lysine + NAD+ = 1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-lysine:NAD+ oxidoreductase (deaminating, cyclizing)
-
CAS REGISTRY NUMBER
COMMENTARY hide
68073-29-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain IFO 3160
-
-
Manually annotated by BRENDA team
strain IFO 3160
-
-
Manually annotated by BRENDA team
strain SBUG 182
-
-
Manually annotated by BRENDA team
strain SBUG 182
-
-
Manually annotated by BRENDA team
strain IFO 12071
-
-
Manually annotated by BRENDA team
strain IFO 12071
-
-
Manually annotated by BRENDA team
strain ICR 2210
-
-
Manually annotated by BRENDA team
strains ICR 0220, IFO 3310 and IFO 12059
-
-
Manually annotated by BRENDA team
Bacterium cadaveris strain IFO 3731
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain IFO 3764
-
-
Manually annotated by BRENDA team
strain IFO 3764
-
-
Manually annotated by BRENDA team
strain IFO 3525
-
-
Manually annotated by BRENDA team
strain IFO 3525
-
-
Manually annotated by BRENDA team
strain ICR 1820
-
-
Manually annotated by BRENDA team
strain ICR 1820
-
-
Manually annotated by BRENDA team
strain IFO 3848
-
-
Manually annotated by BRENDA team
strain IFO 3848
-
-
Manually annotated by BRENDA team
no activity in Achromobacter superficialis
strain ICR 0890
-
-
Manually annotated by BRENDA team
no activity in Achromobacter superficialis ICR 0890
strain ICR 0890
-
-
Manually annotated by BRENDA team
no activity in Aerobacter aerogenes
strain IFO 3320
-
-
Manually annotated by BRENDA team
no activity in Aerobacter aerogenes IFO 3320
strain IFO 3320
-
-
Manually annotated by BRENDA team
no activity in Arthrobacter tumescens
strain IFO 12960
-
-
Manually annotated by BRENDA team
no activity in Arthrobacter tumescens IFO 12960
strain IFO 12960
-
-
Manually annotated by BRENDA team
no activity in Bacillus cereus
strain IFO 3001
-
-
Manually annotated by BRENDA team
no activity in Bacillus cereus IFO 3001
strain IFO 3001
-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis
strain IFO 12200
-
-
Manually annotated by BRENDA team
no activity in Bacillus licheniformis IFO 12200
strain IFO 12200
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium
strain ICR 1340
-
-
Manually annotated by BRENDA team
no activity in Bacillus megaterium ICR 1340
strain ICR 1340
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis
strain IFO 3037
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis IFO 3037
strain IFO 3037
-
-
Manually annotated by BRENDA team
no activity in Bos taurus
liver homogenate
-
-
Manually annotated by BRENDA team
no activity in Brevibacterium divaricatum
strain ICR 4100
-
-
Manually annotated by BRENDA team
no activity in Brevibacterium divaricatum ICR 4100
strain ICR 4100
-
-
Manually annotated by BRENDA team
no activity in Candida albicans
strains IFO 0197 and 0601
-
-
Manually annotated by BRENDA team
no activity in Candida tropicalis
strain IFO 1401
-
-
Manually annotated by BRENDA team
no activity in Candida tropicalis IFO 1401
strain IFO 1401
-
-
Manually annotated by BRENDA team
no activity in Corynebacterium glutamicum
strain ATCC 13032
-
-
Manually annotated by BRENDA team
no activity in Cryptococcus albidus
strain IFO 0410
-
-
Manually annotated by BRENDA team
no activity in Cryptococcus albidus IFO 0410
strain IFO 0410
-
-
Manually annotated by BRENDA team
no activity in Endomycopsis burtonii
strains IFO 1196 and 0844
-
-
Manually annotated by BRENDA team
no activity in Erwinia aroideae
strain IFO 3830
-
-
Manually annotated by BRENDA team
no activity in Erwinia aroideae IFO 3830
strain IFO 3830
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
no activity in Flavobacterium lutescens
strain IFO 3014
-
-
Manually annotated by BRENDA team
no activity in Flavobacterium lutescens IFO 3014
strain IFO 3014
-
-
Manually annotated by BRENDA team
no activity in Haemonchus contortus
-
-
-
Manually annotated by BRENDA team
no activity in Hansenula anomala
strain 0118
-
-
Manually annotated by BRENDA team
no activity in Hansenula anomala 118
strain 0118
-
-
Manually annotated by BRENDA team
no activity in Hansenula beijerinckii
strain IFO 0981
-
-
Manually annotated by BRENDA team
no activity in Hansenula beijerinckii IFO 0981
strain IFO 0981
-
-
Manually annotated by BRENDA team
no activity in Hansenula bimundalis
strain IFO 1366
-
-
Manually annotated by BRENDA team
no activity in Hansenula bimundalis IFO 1366
strain IFO 1366
-
-
Manually annotated by BRENDA team
no activity in Hansenula canadenis
strain IFO 0937
-
-
Manually annotated by BRENDA team
no activity in Hansenula canadenis IFO 0937
strain IFO 0937
-
-
Manually annotated by BRENDA team
no activity in Hansenula hansenii
strain IFO 0794
-
-
Manually annotated by BRENDA team
no activity in Hansenula hansenii IFO 0794
strain IFO 0794
-
-
Manually annotated by BRENDA team
no activity in Hansenula holstii
strain IFO 0980
-
-
Manually annotated by BRENDA team
no activity in Hansenula holstii IFO 0980
strain IFO 0980
-
-
Manually annotated by BRENDA team
no activity in Hansenula mrakii
strain IFO 0895
-
-
Manually annotated by BRENDA team
no activity in Hansenula mrakii IFO 0895
strain IFO 0895
-
-
Manually annotated by BRENDA team
no activity in Hansenula pettersonii
strain IFO 1372
-
-
Manually annotated by BRENDA team
no activity in Hansenula pettersonii IFO 1372
strain IFO 1372
-
-
Manually annotated by BRENDA team
no activity in Hansenula saturnus
strain IFO 0125 and 0992
-
-
Manually annotated by BRENDA team
no activity in Hansenula saturnus IFO 0125
strain IFO 0125 and 0992
-
-
Manually annotated by BRENDA team
no activity in Kluyveromyces lactis
strain IFO 1090
-
-
Manually annotated by BRENDA team
no activity in Kluyveromyces lactis IFO 1090
strain IFO 1090
-
-
Manually annotated by BRENDA team
no activity in Oosporidium margaritiferum
strain IFO 1208
-
-
Manually annotated by BRENDA team
no activity in Oosporidium margaritiferum IFO 1208
strain IFO 1208
-
-
Manually annotated by BRENDA team
no activity in Pichia polymorpha
strain IFO 0195
-
-
Manually annotated by BRENDA team
no activity in Pichia polymorpha IFO 0195
strain IFO 0195
-
-
Manually annotated by BRENDA team
no activity in Proteus vulgaris
strain IFO 3167
-
-
Manually annotated by BRENDA team
no activity in Proteus vulgaris IFO 3167
strain IFO 3167
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas aerofaciens
strain IFO 3521
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas aerofaciens IFO 3521
strain IFO 3521
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas aeruginosa
strain IFO 3080
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas aeruginosa IFO 3080
strain IFO 3080
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas dacunhae
strain ICR 3180
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas dacunhae ICR 3180
strain ICR 3180
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens
strain IFO 3081
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas fluorescens IFO 3081
strain IFO 3081
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas marginalis
strain IFO 3925
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas marginalis IFO 3925
strain IFO 3925
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas ovalis
strain IFO 3738
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas ovalis IFO 3738
strain IFO 3738
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula glutinis
strain IFO 0898
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula glutinis IFO 0898
strain IFO 0898
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula lactosa
strain IFO 1006
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula lactosa IFO 1006
strain IFO 1006
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula marina
strain IFO 0879
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula marina IFO 0879
strain IFO 0879
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula minuta
strain IFO 0387
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula minuta IFO 0387
strain IFO 0387
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula rubra
strains IFO 0001, 0003, 0870 and 0900
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula texensis var. minuta
strain IFO 0412
-
-
Manually annotated by BRENDA team
no activity in Rhodotorula texensis var. minuta IFO 0412
strain IFO 0412
-
-
Manually annotated by BRENDA team
no activity in Sarcina subflava
strain IFO 11992
-
-
Manually annotated by BRENDA team
no activity in Sarcina subflava IFO 11992
strain IFO 11992
-
-
Manually annotated by BRENDA team
no activity in Serratia marcescens
strain IFO 3046
-
-
Manually annotated by BRENDA team
no activity in Serratia marcescens IFO 3046
strain IFO 3046
-
-
Manually annotated by BRENDA team
no activity in Staphylococcus aureus
strain IFO 3060
-
-
Manually annotated by BRENDA team
no activity in Staphylococcus aureus IFO 3060
strain IFO 3060
-
-
Manually annotated by BRENDA team
no activity in Teladorsagia circumcincta
-
-
-
Manually annotated by BRENDA team
no activity in Torolupsis xylinus
strain IFO 0454
-
-
Manually annotated by BRENDA team
no activity in Torolupsis xylinus IFO 0454
strain IFO 0454
-
-
Manually annotated by BRENDA team
no activity in Torulopsis candida
strains IFO 0380 and 0768
-
-
Manually annotated by BRENDA team
no activity in Trichosporon cutaneum
strains IFO 0174 and 1198
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica
strain IFO 1209
-
-
Manually annotated by BRENDA team
no activity in Yarrowia lipolytica IFO 1209
strain IFO 1209
-
-
Manually annotated by BRENDA team
strain IFO 3458
-
-
Manually annotated by BRENDA team
strain IFO 3458
-
-
Manually annotated by BRENDA team
gene lysdh
-
-
Manually annotated by BRENDA team
strain IFO 12670
-
-
Manually annotated by BRENDA team
strain IFO 12670
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
genomic organization and functional role of lysine catabolic pathways among prokaryotes, overview
physiological function
-
the enzyme is part of the lysine-to alpha-aminoadipic-delta-semialdehyde-pathway, that has a broad evolutionary importance in osmotic stress resistance
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxylysine + NADP+
alpha-amino-gamma-hydroxyadipate-semialdehyde + NADPH
show the reaction diagram
5-hydroxylysine + NADP+
alpha-amino-delta-hydroxyadipate-semialdehyde + NADPH
show the reaction diagram
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH
show the reaction diagram
-
-
-
?
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
-
-
-
-
r
L-lysine + NAD+ + H2O
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
show the reaction diagram
S-(2-aminoethyl)-L-cysteine + NADP+ + H2O
S-(2-oxoethyl)-L-cysteine + NADPH + NH3
show the reaction diagram
additional information
?
-
-
LysDH directly converts L-lysine to alpha-aminoadipic-delta-semialdehyde
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH
show the reaction diagram
-
-
-
?
L-lysine + NAD+
1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+
show the reaction diagram
-
-
-
-
r
L-lysine + NAD+ + H2O
1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
show the reaction diagram
-
epsilon amino group is oxidatively deaminated to alpha-aminoadipate delta-semialdehyde, which is spontaneously converted into 1,2-didehydropiperidine-6-carboxylate, first step of lysine degradation
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5-dithiobis(2-nitrobenzoate)
-
0.5 mM, 70% inhibition, 40% inhibition after preincubation with 10 mM L-lysine, 98.5% inhibition after preincubation with 1 mM NAD+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-Aminovalerate
-
5 mM, 1.65fold activation
6-aminocaproate
-
5 mM, 2.32fold activation
7-aminoheptanoate
-
5 mM, 2.3fold activation
8-aminooctanoate
-
5 mM, 2.26fold activation
D-Lysine
-
5 mM, 1.58fold activation
D-phenylalanine
-
5 mM, 1.23fold activation
D-tryptophan
-
5 mM, 2.01fold activation
DL-alpha-hydroxy-n-caproate
-
5 mM, 1.85fold activation
DL-Homolysine
-
5 mM, 1.8fold activation
L-isoleucine
-
5 mM, 1.72fold activation
L-leucine
-
5 mM, 1.69fold activation
L-lysine
-
5 mM, 2.29fold activation
L-methionine
-
5 mM, 1.54fold activation
L-norleucine
-
5 mM, 2.05fold activation
L-norvaline
-
5 mM, 1.71fold activation
L-phenylalanine
-
5 mM, 2.09fold activation
L-phenylglycine
-
5 mM, 1.74fold activation
L-Phenyllactate
-
5 mM, 2.25fold activation
L-tryptophan
-
5 mM, 1.24fold activation
L-tyrosine
-
5 mM, 1.41fold activation
L-valine
-
5 mM, 1.47fold activation
S-(2-aminoethyl)-L-cysteine
-
5 mM, 2.32fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.13
3-acetylpyridine-NAD+
-
-
1.85
deamino-NAD+
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00004
-
enzyme activity in crude extracts
0.000059
-
enzyme activity in crude extracts
0.000069
-
enzyme activity in crude extracts
0.0001
-
enzyme activity in crude extracts
0.00017
-
enzyme activity in crude extracts, strain IFO 3319
0.00021
-
enzyme activity in crude extracts
0.00026
-
enzyme activity in crude extracts
0.00067
-
enzyme activity in crude extracts
0.00069
-
enzyme activity in crude extracts
0.0016
-
enzyme activity in crude extracts
0.0029
-
enzyme activity in crude extracts
0.0052
-
enzyme activity in crude extracts, strain ICR 0220
0.0144
-
activity in cells grown on 5 mM lysine
0.0392
-
enzyme activity in crude extracts, strain IFO 12059
0.0696
-
enzyme activity in crude extracts, strain IFO 3058
0.0739
-
enzyme activity in crude extracts, strain ICR 1600
additional information
-
0.874 micromol/min*l homogenate, 3 months old baby girl with congenital lysine intolerance, in the absence of cysteine; 11.96 micromol/min*l homogenate, in the presence of cysteine; 2.70 micromol/min*l homogenate, 3 months old baby girl with congenital lysine intolerance, in the presence of cysteine; 6.24 micromol/min*l homogenate, in the absence of cysteine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
oxidative deamination of S-(beta-aminoethyl)-cysteine, 5- and 4-hydroxylysine
9.3
-
oxidative deamination of lysine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
-
with S-(2-aminoethyl)-cysteine and 5-hydroxylysine
37
-
with lysine and 4-hydroxylysine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
in the absence of lysine, approx. 50% activity after 10 min at 40°C
30 - 60
-
in the presence of 5 mM L-lysine, approx. 50% activity after 10 min at 55°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene lysdh is not upregulated under salt stress, in contrast to several other enzymes from the lysine-to-alpha-aminoadipic-delta-semialdehyde pathways, but is upregulated by addition of exogenous lysine
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
amperometric biosensor for L-lysine based on immobilized enzyme