Information on EC 2.1.1.255 - geranyl diphosphate 2-C-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.255
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RECOMMENDED NAME
GeneOntology No.
geranyl diphosphate 2-C-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + geranyl diphosphate = S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-methylisoborneol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:geranyl-diphosphate 2-C-methyltransferase
This enzyme, along with EC 4.2.3.118, 2-methylisoborneol synthase, produces 2-methylisoborneol, an odiferous compound produced by soil microorganisms with a strong earthy/musty odour.
CAS REGISTRY NUMBER
COMMENTARY hide
57219-74-6
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (E)-2-methylgeranyl diphosphate
S-adenosyl-L-homocysteine + 2-methylisoborneol
show the reaction diagram
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?
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
show the reaction diagram
additional information
?
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SCO7701 shows no activity with with farnesyl diphosphate or geranylgeranyl diphosphate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + geranyl diphosphate
S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
(E)-2-methylgeranyl diphosphate
pH 7.0, 37C, recombinant enzyme
0.0131
geranyl diphosphate
pH 7.0, 37C, recombinant enzyme; pH and temperature not specified in the publication
0.0043
S-adenosyl-L-methionine
pH 7.0, 37C, recombinant enzyme; pH and temperature not specified in the publication
additional information
additional information
steady-state kinetics, Michaelis-Menten kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.039
(E)-2-methylgeranyl diphosphate
Streptomyces coelicolor
Q9F1Y5
pH 7.0, 37C, recombinant enzyme
0.0075
geranyl diphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 49937, recombinant N-terminally His6-tagged soluble SCO7700, mass spectrometry, x * 49955, sequence calculation
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged wild-type enzyme in complex with S-adenosyl-L-methionine and monoterpenoid ligands geranyl-S-thiolodiphosphate, a substrate analogue, or geranyl diphosphate, sitting drop vapor diffusion method, 4C, mixing of 0.001 ml protein solution conatining 6 mg/mL enzyme, 50 mM PIPES, pH 6.7, 20% glycerol, 5 mM BME, 15 mM MgCl2, 100 mM NaCl, 10 mM S-adenosyl-L-methionine, and 1 mM monoterpenoid ligand with 0.001 ml of precipitant solution containing 100 mM HEPES, pH 7.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl-S-thiolodiphosphate, 100 mM Bis-Tris, pH 6.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl diphosphate and equilibrated against a 0.1 ml reservoir of precipitant solution at 21C, 1 day, X-ray diffraction structure determination and analysis at 2.05 A resolution
purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method; sitting-drop vapour-diffusion method
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
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recombinant N-terminally His6-tagged soluble SCO7700 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity and over 95% purity
soluble recombinant His6-tagged wild-type and mutant geranyl diphosphate methyltransferase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichia coli; expression in Escherichia coli as N-terminal His6-tagged protein
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis and tree, expression in Escherichia coli; expression in Escherichia coli
expressed in Escherichia coli; gene sco7700, DNA and amino acid sequence determination and analysis, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in Escherichia coli as N-terminal His6-tagged protein
expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3); overproduced in Escherichia coli strain BL21
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gene pgmtf, real-time-RT PCR enzyme expression analysis
wild-type and mutant geranyl diphosphate methyltransferase expression as His6-tagged enzyme in Escherichia coli strain BL21(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression level of the gene encoding GPPMT decreases at 4C and increased at 30C, compared with the level at 20C
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE