Information on EC 2.1.2.7 - D-alanine 2-hydroxymethyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.2.7
-
RECOMMENDED NAME
GeneOntology No.
D-alanine 2-hydroxymethyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,10-methylenetetrahydrofolate + D-alanine + H2O = tetrahydrofolate + 2-methylserine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxymethyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
One carbon pool by folate
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:D-alanine 2-hydroxymethyltransferase
Also acts on 2-hydroxymethylserine.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-76-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K12
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
strain MS, inducible enzyme
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-L-serine + tetrahydrofolate
D-alanine + 5,10-methylenetetrahydrofolate
show the reaction diagram
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
show the reaction diagram
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
show the reaction diagram
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
at higher concentrations
DL-cycloserine
-
strong, competitive
Fe2+
-
at higher concentrations
Fe3+
-
at higher concentrations
L-Cycloserine
-
-
L-serine
-
weak, competitive
p-chloromercuribenzoate
-
-
Zn2+
-
at higher concentrations
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.2
2-ethyl-DL-serine
-
pH 7.5
1.5
2-methyl-DL-serine
1.88
2-methyl-L-serine
-
0.8
hydroxymethylserine
-
pH 7.5
0.25
L-tetrahydrofolate
-
pH 7.5, calculated on the basis of one isomer
0.09 - 0.228
tetrahydrofolate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 0.0022
D-cycloserine
0.005
L-Cycloserine
-
-
5.6
L-serine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
-
about half-maximal activity at pH 6.0 and 10.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33 - 48
-
about half-maximal activity at 33C and 90% activity at 48C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
73000
gel filtration
80000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
702735
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
96% loss of activity after 10 min, hydroxymethylserine plus tetrahydrofolate and pyridoxal 5'-phosphate prevent
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
prolonged dialysis inactivates
-
pyridoxal 5-phosphate restores activity
-
pyridoxal 5-phosphate stabilizes during purification
-
tetrahydrofolate plus pyridoxal 5-phosphate plus hydroxymethylserine increase temperature-stability
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
column chromatography, 16.4% yield
column chromatography, 72.8% yield
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli