Information on EC 2.5.1.128 - N4-bis(aminopropyl)spermidine synthase

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The expected taxonomic range for this enzyme is: Thermococcus kodakarensis

EC NUMBER
COMMENTARY hide
2.5.1.128
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RECOMMENDED NAME
GeneOntology No.
N4-bis(aminopropyl)spermidine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 S-adenosyl 3-(methylthio)propylamine + spermidine = 2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermidine = S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + spermidine = S-methyl-5'-thioadenosine + N4-aminopropylspermidine
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
polyamine pathway
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl 3-(methylthio)propylamine:spermidine 3-aminopropyltransferase (N4-bis(aminopropyl)spermidine synthesizing)
The enzyme, characterized from the thermophilic archaeon Thermococcus kodakarensis, synthesizes the branched-chain polyamine N4-bis(aminopropyl)spermidine, which is required for cell growth at high-temperature. When spermine is used as substrate, the enzyme forms N4-aminopropylspermine.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
at 85C the bspA disruptant strain shows a slightly extended lag phase compared to that of the wild-type strain. At 93C there is no cell growth of the disruptant strain. The bpsA gene is required for growth at the higher temperature. The growth defect of the disruptant strain at 93C is partially restored by the addition of 1 mM N4-bis(aminopropyl)spermidine to the medium. N4-bis(Aminopropyl)spermidine is required for cell growth of Thermococcus kodakarensis in a higher-temperature environment
physiological function
N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93C
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + spermidine
2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
S-adenosyl 3-(methylthio)propylamine + N4-aminopropylspermine
S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
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-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + N4-aminopropylspermidine
show the reaction diagram
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-
-
?
S-adenosyl 3-(methylthio)propylamine + spermine
S-methyl-5'-thioadenosine + N4-aminopropylspermine
show the reaction diagram
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl 3-(methylthio)propylamine + spermidine
2 S-methyl-5'-thioadenosine + N4-bis(aminopropyl)spermidine
show the reaction diagram
Q5JIZ3
N4-bis(aminopropyl)spermidine is the major polyamine in Thermococcus kodakarensis. The branched-chain polyamine synthesized by BpsA is important for cell growth at 93C
overall reaction
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?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
pH 9.0, 70C, production of N4-aminopropylspermine
0.34
pH 9.0, 70C, production of N4-bis(aminopropyl)spermidine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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