Information on EC 2.5.1.4 - adenosylmethionine cyclotransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.4
-
RECOMMENDED NAME
GeneOntology No.
adenosylmethionine cyclotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine = S-methyl-5'-thioadenosine + 2-aminobutan-4-olide
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleophilic substitution
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine alkyltransferase (cyclizing)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-34-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Sprague-Dawley rat
-
-
Manually annotated by BRENDA team
pig
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine
5'-methylthioadenosine + 2-aminobutan-4-olide
show the reaction diagram
S-adenosyl-L-methionine
5'-methylthioadenosine + alpha-amino-gamma-butyrolactone
show the reaction diagram
S-adenosylethionine
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine
5'-methylthioadenosine + alpha-amino-gamma-butyrolactone
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor is required
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-O-Methyladenosine
-
weak inhibition
adenine
-
weak inhibition
adenosine
-
weak inhibition
Allo-S-Adenosyl-L-methionine
-
4 mM inhibits more than 50%
-
dimethyladenosylsulfonium
-
weak inhibition
ethylthioadenosine
-
4 mM inhibits more than 50%
L-methionine
-
weak inhibition
methionine methylsulfonium
-
weak inhibition
methylthioadenosine
-
4 mM inhibits more than 50%
Methylthioinosine
-
weak inhibition
S-adenosyl-L-ethionine
S-adenosyl-L-homocysteine
S-Ribosylmethionine
SH-binding reagents
-
-
-
additional information
-
no effect: Mg2+, EDTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 1
S-adenosyl-L-methionine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
S-adenosyl-L-homocysteine
-
competitive inhibitor to S-adenosylmethionine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.061
-
-
0.099
-
porcine liver homogenate supernatant
0.108
-
pH 5 preparation
0.117
-
ammonium sulfate-treated, pH 5 enzyme preparation
3.07
-
E. coli infected with bacteriophage T3
28.3
-
E. coli infected with bacteriophage UV-T3
additional information
-
synthesized enzyme tested according to the method of Gefter et al.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
about 60% of activity maximum at pH 6 and 9
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cells
Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not stable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, enzyme from E. coli infected with bacteriophage UV-T3 is stable for 2 weeks
-
0°C or -10°C, enzyme from E. coli infected with bacteriophage T3 loses all of its activity within 3 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
method that includes chromatography on DEAE-cellulose
-
partial purification by a method that includes precipitation with acetone, differential heat inactivation and precipitation by ammonium sulfate
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