Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
L117P | mutant enzyme without activity | Streptomyces sp. |
L119A | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
L119F | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
P357N | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
Q286R | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
S279R | mutant enzyme without activity on cholesterol and pregnenolone | Streptomyces sp. |
S379D | mutant enzyme without activity on cholesterol and pregnenolone | Streptomyces sp. |
S379T | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
V145Q | specific activity is similar to that of the wild-type enzyme | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000058 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379T | Streptomyces sp. | |
0.0000081 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379T | Streptomyces sp. | |
0.00002 | - |
cholesterol | pH 7.0, 37°C, wild-type enzyme | Streptomyces sp. | |
0.000023 | - |
pregnenolone | pH 7.0, 37°C, wild-type enzyme | Streptomyces sp. | |
0.000023 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379A | Streptomyces sp. | |
0.00003 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379A | Streptomyces sp. | |
0.00004 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme L119A | Streptomyces sp. | |
0.00004 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379V | Streptomyces sp. | |
0.000046 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379V | Streptomyces sp. | |
0.000056 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme Q286R | Streptomyces sp. | |
0.000058 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145Q | Streptomyces sp. | |
0.000072 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145Q | Streptomyces sp. | |
0.000077 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme L119F | Streptomyces sp. | |
0.000081 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme L119A | Streptomyces sp. | |
0.000083 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145E | Streptomyces sp. | |
0.000098 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme P357N | Streptomyces sp. | |
0.0001 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145D | Streptomyces sp. | |
0.00011 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme P357N | Streptomyces sp. | |
0.00013 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme Q286R | Streptomyces sp. | |
0.00017 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme L119F | Streptomyces sp. | |
0.0004 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145D | Streptomyces sp. | |
0.00067 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145E | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, wild-type and mutant enzymes V145Q, V145E, S379A and S379V, SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes V145Q, V145E, S379A and S379V | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-5-en-3beta-ol 7-one + O2 | low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T | Streptomyces sp. | cholest-5-en-3,17-dione + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
dehydroisoandrosterone + O2 | low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T | Streptomyces sp. | androst-5-en-3,17-dione + H2O | - |
? | |
dihydrocholesterol + O2 | - |
Streptomyces sp. | 5alpha-cholestan-3-one + H2O2 | - |
? | |
estrone + O2 | low activity with wild-type enzyme, no activity with mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T | Streptomyces sp. | estra-1,5(10)-dien-3,17-dione + H2O2 | - |
? | |
pregnenolone + O2 | - |
Streptomyces sp. | 5-pregnen-3,20-dione + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, wild-type and mutant enzymes V145Q, V145E, S379A and S379V, SDS-PAGE | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme P357N | Streptomyces sp. | |
9 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme P357N | Streptomyces sp. | |
9 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145D | Streptomyces sp. | |
11 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145D | Streptomyces sp. | |
11 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145E | Streptomyces sp. | |
11 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145Q | Streptomyces sp. | |
12 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme V145Q | Streptomyces sp. | |
13 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme L119A | Streptomyces sp. | |
15 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme L119A | Streptomyces sp. | |
19 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme Q286R | Streptomyces sp. | |
21 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme L119F | Streptomyces sp. | |
23 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme Q286R | Streptomyces sp. | |
23 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme V145E | Streptomyces sp. | |
27 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme L119F | Streptomyces sp. | |
29 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379T | Streptomyces sp. | |
30 | - |
pregnenolone | pH 7.0, 37°C, wild-type enzyme | Streptomyces sp. | |
35 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379A | Streptomyces sp. | |
39 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379V | Streptomyces sp. | |
40 | - |
cholesterol | pH 7.0, 37°C, wild-type enzyme | Streptomyces sp. | |
45 | - |
pregnenolone | pH 7.0, 37°C, mutant enzyme S379T | Streptomyces sp. | |
49 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379A | Streptomyces sp. | |
51 | - |
cholesterol | pH 7.0, 37°C, mutant enzyme S379V | Streptomyces sp. |