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Literature summary for 1.1.3.6 extracted from

  • Gadbery, J.E.; Sampson, N.S.
    Use of an isotope-coded mass tag (ICMT) method to determine the orientation of cholesterol oxidase on model membranes (2018), Biochemistry, 57, 5370-5378 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.6

Protein Variants

Protein Variants Comment Organism
A184C/A301C/T394C kcat/KM is comparable to wild-type value Streptomyces sp. SA-COO
A184C/T239C/A407C/A465C kcat/KM is 1.1fold lower than wild-type value Streptomyces sp. SA-COO
A32C/S129C/T371C/A423C kcat/KM is 2.55fold lower than wild-type value Streptomyces sp. SA-COO
A32C/T168C/S312C/A465C kcat/KM is 2.1fold lower than wild-type value Streptomyces sp. SA-COO
L274C kcat/KM is 1.13fold lower than wild-type value Streptomyces sp. SA-COO
L80C kcat/KM is 1.6fold higher than wild-type value Streptomyces sp. SA-COO
S153C/A205C/S312C/T435C kcat/KM is 1.4fold higher than wild-type value Streptomyces sp. SA-COO
T168C/A276C kcat/KM is 1.5fold lower than wild-type value Streptomyces sp. SA-COO

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 cholesterol pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.011
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.011
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.011
-
 cholesterol pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.012
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.013
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.013
-
 cholesterol pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.014
-
 cholesterol pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
0.017
-
 cholesterol pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO

Organism

Organism UniProt Comment Textmining
Streptomyces sp. SA-COO P12676
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp. SA-COO

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Streptomyces sp. SA-COO cholest-5-en-3-one + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
30
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
46
-
 cholesterol pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
57
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
60
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
67
-
 cholesterol pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
68
-
 cholesterol pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
75
-
 cholesterol pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
89
-
 cholesterol pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO

General Information

General Information Comment Organism
drug target cholesterol oxidase can alter the structure of the cell membrane in pathogenic bacteria and is thus a potential antimicrobial drug target Streptomyces sp. SA-COO

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2000
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
2400
-
 cholesterol pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
3400
-
 cholesterol pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
4500
-
 cholesterol pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
4600
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
5000
-
 cholesterol pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
5100
-
 cholesterol pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
7000
-
 cholesterol pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO
8400
-
 cholesterol pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate Streptomyces sp. SA-COO