Protein Variants | Comment | Organism |
---|---|---|
F70A | improved activities toward cholestane skeleton substrates. Improved activity toward beta-sitosterol, dehydroepiandrosterone, pregnenolone and stigmasterol | Pimelobacter simplex |
F70L | the mutant enzyme shows almost the same level of activity as the wild-type enzyme | Pimelobacter simplex |
F70V | mutation increases the binding free energy between the mutant enzyme (PsChO) and cholesterol and might cause the movement of loops L56-P77, K45-P49 and L350-E354 at active site. Improved activities toward cholestane skeleton substrates. 50% higher activities toward cholesterol when compared with that of the wild-type enzyme | Pimelobacter simplex |
V64C | mutation increases the binding free energy between the mutant enzyme (PsChO) and cholesterol and might cause the movement of loops L56-P77, K45-P49 and L350-E354 at active site. Improved activities toward cholestane skeleton substrates. 50% higher activities toward cholesterol when compared with that of the wild-type enzyme | Pimelobacter simplex |
V64I | improved activities toward cholestane skeleton substrates. Improved activity toward beta-sitosterol, dehydroepiandrosterone, pregnenolone and stigmasterol | Pimelobacter simplex |
V64L | improved activities toward cholestane skeleton substrates. Improved activity toward beta-sitosterol, dehydroepiandrosterone, pregnenolone and stigmasterol | Pimelobacter simplex |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15483 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
0.17831 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
0.17842 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
0.19061 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
0.20485 | - |
cholesterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
0.22076 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
0.22842 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
0.27352 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
0.29455 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
0.31881 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
0.32861 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
0.34121 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
0.38412 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
0.52884 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
0.78961 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pimelobacter simplex | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + O2 | - |
Pimelobacter simplex | ? + H2O2 | - |
? | |
cholesterol + O2 | - |
Pimelobacter simplex | cholest-5-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | - |
Pimelobacter simplex | androst-5-en-3,17-dione + H2O2 | - |
? | |
pregnenolone + O2 | - |
Pimelobacter simplex | 5-pregnen-3,20-dione + H2O2 | - |
? | |
stigmasterol + O2 | - |
Pimelobacter simplex | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pimelobacter simplex |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, 25% residual activity | Pimelobacter simplex |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
1.58 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
4.23 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
6.82 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
9.56 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
9.86 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
11.78 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
12.29 | - |
cholesterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
13.25 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
13.68 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
13.71 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
15.25 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
15.48 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
16.06 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
23.02 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pimelobacter simplex |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 75% of the maximal activity is retained over the pH range of 5.0-9.0 | Pimelobacter simplex |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | good pH stability over the pH range of 5.0-9.0 | Pimelobacter simplex |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
8 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
20 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
20 | - |
stigmasterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
30 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
30 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
40 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
50 | - |
dehydroepiandrosterone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
60 | - |
cholesterol | pH 7.5, temperature not specified in the publication, wild-type enzyme | Pimelobacter simplex | |
60 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
60 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex | |
80 | - |
pregnenolone | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
90 | - |
beta-sitosterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
100 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme F70V | Pimelobacter simplex | |
130 | - |
cholesterol | pH 7.5, temperature not specified in the publication, mutant enzyme V64C | Pimelobacter simplex |