Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Sinorhizobium meliloti |
53190 | - |
predicted from the pdxR DNA sequence | Sinorhizobium meliloti |
106000 | - |
gel filtration chromatography | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
formerly Rhizobium meliloti, strain IFO 14782 | - |
Purification (Comment) | Organism |
---|---|
Q Sepharose FF column chromatography and Sephadex LH 20 column chromatography | Sinorhizobium meliloti |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.946 | - |
0.091 mM DCIP and 0.00024 mg purified enzyme in 33 mM potassium phosphate buffer at pH 7.0 | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phospho-D-erythronate + 2,6-dichloroindophenol | - |
Sinorhizobium meliloti | 3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + reduced 2,6-dichloroindophenol | - |
? | |
4-phospho-D-erythronate + FAD | - |
Sinorhizobium meliloti | 3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + FADH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 53000, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
4PE dehydrogenase | - |
Sinorhizobium meliloti |
4PE oxidoreductase | - |
Sinorhizobium meliloti |
flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase | - |
Sinorhizobium meliloti |
pdxR-encoded 4PE dehydrogenase | - |
Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sinorhizobium meliloti |