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Literature summary for 1.13.11.49 extracted from

  • Schmidt, D.; Serra, I.; Mlynek, G.; Pfanzagl, V.; Hofbauer, S.; Furtmueller, P.G.; Djinovic-Carugo, K.; Van Doorslaer, S.; Obinger, C.
    Arresting the catalytic arginine in chlorite dismutases Impact on heme coordination, thermal stability, and catalysis (2021), Biochemistry, 60, 621-634 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Gold (DE3) cells Cyanothece sp. PCC 7425

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes Q74V and Q74E, sitting drop vapor diffusion method, using 0.1 M MES (pH 6.5), 0.15 M MgSO4, 28% (w/v) polyethylene glycol 3350, and 3% (v/v) glycerol Cyanothece sp. PCC 7425

Protein Variants

Protein Variants Comment Organism
Q74E the catalytic efficiency (kcat/KM) of chlorite degradation of the variant is similar to that of the wild type protein Cyanothece sp. PCC 7425
Q74V the catalytic efficiency (kcat/KM) of chlorite degradation of the variant is similar to that of the wild type protein Cyanothece sp. PCC 7425

Inhibitors

Inhibitors Comment Organism Structure
Chlorite above 0.1 mM chlorite (5000fold excess) irreversible inactivation is observed Cyanothece sp. PCC 7425

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0188
-
 Chlorite mutant enzyme Q74V, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
0.0193
-
 Chlorite mutant enzyme Q74V, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
0.021
-
 Chlorite mutant enzyme Q74E, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
0.0432
-
 Chlorite wild type enzyme, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
0.0478
-
 Chlorite mutant enzyme Q74V, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
0.0538
-
 Chlorite mutant enzyme Q74E, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
0.0592
-
 Chlorite wild type enzyme, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
0.1271
-
 Chlorite wild type enzyme, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
0.327
-
 Chlorite mutant enzyme Q74E, at pH 5.0 and 30°C Cyanothece sp. PCC 7425

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorite Cyanothece sp. PCC 7425
-
 chloride + O2
-
 ?

Organism

Organism UniProt Comment Textmining
Cyanothece sp. PCC 7425 B8HNS6
-
-

Purification (Commentary)

Purification (Comment) Organism
His-trap affinity column chromatography and Superdex 200 gel filtration Cyanothece sp. PCC 7425

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorite
-
 Cyanothece sp. PCC 7425 chloride + O2
-
 ?

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
 Cyanothece sp. PCC 7425
CLD
-
 Cyanothece sp. PCC 7425

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.91
-
 Chlorite mutant enzyme Q74E, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
33.4
-
 Chlorite wild type enzyme, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
37.2
-
 Chlorite mutant enzyme Q74E, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
43
-
 Chlorite mutant enzyme Q74V, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
50.8
-
 Chlorite mutant enzyme Q74V, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
60
-
 Chlorite wild type enzyme, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
280.2
-
 Chlorite mutant enzyme Q74V, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
475.8
-
 Chlorite wild type enzyme, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
977.4
-
 Chlorite mutant enzyme Q74E, at pH 5.0 and 30°C Cyanothece sp. PCC 7425

Cofactor

Cofactor Comment Organism Structure
heme b
-
 Cyanothece sp. PCC 7425

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
560
-
 Chlorite wild type enzyme, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
690
-
 Chlorite mutant enzyme Q74E, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
900
-
 Chlorite mutant enzyme Q74E, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
1400
-
 Chlorite wild type enzyme, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
2200
-
 Chlorite mutant enzyme Q74V, at pH 9.0 and 30°C Cyanothece sp. PCC 7425
2700
-
 Chlorite mutant enzyme Q74V, at pH 7.0 and 30°C Cyanothece sp. PCC 7425
3000
-
 Chlorite mutant enzyme Q74E, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
3700
-
 Chlorite wild type enzyme, at pH 5.0 and 30°C Cyanothece sp. PCC 7425
5900
-
 Chlorite mutant enzyme Q74V, at pH 5.0 and 30°C Cyanothece sp. PCC 7425