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Literature summary for 1.13.11.49 extracted from

  • Mahor, D.; Pueschmann, J.; van den Haak, M.; Kooij, P.J.; van den Ouden, D.L.J.; Strampraad, M.J.F.; Srour, B.; Hagedoorn, P.L.
    A traffic light enzyme acetate binding reversibly switches chlorite dismutase from a red- to a green-colored heme protein (2020), J. Biol. Inorg. Chem., 25, 609-620 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Azospira oryzae

Protein Variants

Protein Variants Comment Organism
R183a the mutation results in the loss of the ability to bind acetate Azospira oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorite Azospira oryzae
-
 chloride + O2
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
acetic acid acetate buffer pH 5.0 affects the enzyme stability. Prolonged incubation (2 h) results in a 90% activity loss Azospira oryzae

Organism

Organism UniProt Comment Textmining
Azospira oryzae E2DI02
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography Azospira oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.96
-
 in 100 mM acetate buffer, at pH 5.0 and 20°C Azospira oryzae
8.27
-
 in 100 mM citrate phosphate buffer, at pH 5.0 and 20°C Azospira oryzae
8.52
-
 in 100 mM potassium phosphate buffer, at pH 7.0 and 20°C Azospira oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorite
-
 Azospira oryzae chloride + O2
-
 ?
chlorite the enzyme is highly specific for chlorite Azospira oryzae chloride + O2
-
 ?
additional information the enzyme can reversibly bind acetate to form a green colored high spin ferric complex Azospira oryzae ?
-
-

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Azospira oryzae

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
 Azospira oryzae
CLD
-
 Azospira oryzae

Cofactor

Cofactor Comment Organism Structure
heme
-
 Azospira oryzae