Literature summary for 1.13.11.67 extracted from

Daruwalla, A.; Zhang, J.; Lee, H.J.; Khadka, N.; Farquhar, E.R.; Shi, W.; von Lintig, J.; Kiser, P.D.
Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase (2020), Proc. Natl. Acad. Sci. USA, 117, 19914-19925 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli T7 Express cells	Candidatus Nitrosotalea devanaterra

Crystallization (Commentary)

Crystallization (Comment)	Organism
iron-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaHOH, pH 6.0, 0.1 M NaCl. 22% (v/v) Sokalan PA 25 CL and 22% (w/v) xylitol. Cobalt-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaOH, pH 6.0, 0.1 M NaCl, 22% (v/v) Sokalan PA 25 CL, 5% (v/v) glycerol and 10-13% (w/v) xylitol	Candidatus Nitrosotalea devanaterra

Crystallization (Comment)

Organism

iron-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaHOH, pH 6.0, 0.1 M NaCl. 22% (v/v) Sokalan PA 25 CL and 22% (w/v) xylitol. Cobalt-bound enzyme in complex with 3-hydroxy-beta-apo-12'-carotenal, sitting drop vapor diffusion method, using 0.1 M MES-NaOH, pH 6.0, 0.1 M NaCl, 22% (v/v) Sokalan PA 25 CL, 5% (v/v) glycerol and 10-13% (w/v) xylitol

Candidatus Nitrosotalea devanaterra

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0182	-	all-trans-beta-apo-8'-carotenol	at pH 7.0 and 22°C	Candidatus Nitrosotalea devanaterra
0.0982	-	all-trans-8'-apo-beta-carotenal	at pH 7.0 and 22°C	Candidatus Nitrosotalea devanaterra

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains an iron center	Candidatus Nitrosotalea devanaterra

Organism

Organism	UniProt	Comment	Textmining
Candidatus Nitrosotalea devanaterra	A0A128A3G4	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration	Candidatus Nitrosotalea devanaterra

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3-hydroxy-beta-apo-12'-carotenal + O2	the enzyme cleaves specifically at the C14'-C13' position	Candidatus Nitrosotalea devanaterra	?	-	?
all-trans-8'-apo-beta-carotenal + O2	the enzyme cleaves specifically at the C14'-C13' position	Candidatus Nitrosotalea devanaterra	beta-apo-14'-carotenal + ?	-	?
all-trans-beta-apo-8'-carotenol	-	Candidatus Nitrosotalea devanaterra	14'-apo-carotenal + ?	-	?
beta-apo-10'-carotenal	-	Candidatus Nitrosotalea devanaterra	14'-apo-carotenal + ?	-	?
additional information	no activity with beta-carotene, zeaxanthin, lycopene, retinal, resveratrol and isoeugenol	Candidatus Nitrosotalea devanaterra	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 53817, calculated from amino acid sequence	Candidatus Nitrosotalea devanaterra

Synonyms

Synonyms	Comment	Organism
carotenoid cleavage dioxygenase	-	Candidatus Nitrosotalea devanaterra
CCD	-	Candidatus Nitrosotalea devanaterra

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0025	-	all-trans-beta-apo-8'-carotenol	at pH 7.0 and 22°C	Candidatus Nitrosotalea devanaterra
0.11	-	all-trans-8'-apo-beta-carotenal	at pH 7.0 and 22°C	Candidatus Nitrosotalea devanaterra