Cloned (Comment) | Organism |
---|---|
expression of the phenol hydroxylase gene cluster in Escherichia coli strain JM109 in the absence or presence of the phk gene | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
accessory component PHK of phenol hydroxylase | the accessory component of the phenol hydroxylase mediates inhibition of phenol hydroxylase activity, overview | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | PHK, the accessory component of the phenol hydroxylase, may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
PHK accessory protein | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + NADPH + H+ + O2 | Pseudomonas sp. | - |
catechol + NADP+ + H2O | - |
? | |
phenol + NADPH + H+ + O2 | Pseudomonas sp. OX1 | - |
catechol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. OX1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PHK, the accessory component of the phenol hydroxylase, from Escherichia coli | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + NADPH + H+ + O2 | - |
Pseudomonas sp. | catechol + NADP+ + H2O | - |
? | |
phenol + NADPH + H+ + O2 | monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 | Pseudomonas sp. | catechol + NADP+ + H2O | - |
? | |
phenol + NADPH + H+ + O2 | - |
Pseudomonas sp. OX1 | catechol + NADP+ + H2O | - |
? | |
phenol + NADPH + H+ + O2 | monitoring the production of catechol in a continuous coupled assay with recombinant catechol 2,3-dioxygenase from Pseudomonas sp. OX1 | Pseudomonas sp. OX1 | catechol + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 10235.27, PHK accessory protein, mass spectrometry | Pseudomonas sp. |
More | the phenol hydroxylase complex possesses the PHL, PHN, PHO, PHM, and PHK subunits | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
phenol hydroxylase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
additional information | PHK, the accessory component of the phenol hydroxylase, is neither involved in the catalytic activity of the phenol hydroxylase complex nor required for the assembly of apo-hydroxylase, but may be responsible for enhancing iron incorporation into the active site of the apo-hydroxylase | Pseudomonas sp. |