Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | competitive inhibition, Ki: 0.088 mM | Escherichia coli | |
NADH | competitive inhibition, Ki: 0.025 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Escherichia coli | |
0.029 | - |
NAD+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli B / ATCC 11303 | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli B / ATCC 11303 | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable for 30 min | Escherichia coli |
42 | - |
loss of activity after 10 min | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |