Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Mentha x piperita |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mentha x piperita | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000 | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (+)-pulegone + 2 NADPH + 2 H+ | Mentha x piperita | the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | - |
cv Black Mitcham | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision | Mentha x piperita |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
secretory trichome | secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development | Mentha x piperita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (+)-pulegone + 2 NADPH + 2 H+ | the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
2 (+)-pulegone + 2 NADPH + 2 H+ | the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000 | Mentha x piperita |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the medium-chain dehydrogenase/reductase superfamily | Mentha x piperita |
pulegone reductase | - |
Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Mentha x piperita |