Cloned (Comment) | Organism |
---|---|
expression of a chimeric protein with one Acinetobacter domain in Escherichia coli, domain interactions, subunit interactions | Escherichia coli |
expression of a chimeric protein with one Escherichia coli domain in Escherichia coli, domain interactions, subunit interactions | Acinetobacter calcoaceticus subsp. anitratus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric proteins | Escherichia coli |
additional information | chimeric proteins | Acinetobacter calcoaceticus subsp. anitratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | allosteric | Acinetobacter calcoaceticus subsp. anitratus | |
NADH | allosteric | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
oxaloacetate | wild-type | Acinetobacter calcoaceticus subsp. anitratus | |
0.02 | - |
oxaloacetate | wild-type | Escherichia coli | |
0.11 | - |
acetyl-CoA | wild-type | Acinetobacter calcoaceticus subsp. anitratus | |
0.7 | - |
acetyl-CoA | wild-type | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | mutants, overview | Escherichia coli | |
KCl | mutants, overview | Acinetobacter calcoaceticus subsp. anitratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
alignment of partial amino acid sequence | Escherichia coli |
additional information | - |
alignment of partial amino acid sequence | Acinetobacter calcoaceticus subsp. anitratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus subsp. anitratus | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant chimeric protein from E. coli | Escherichia coli |
recombinant chimeric protein from E. coli | Acinetobacter calcoaceticus subsp. anitratus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
influence of KCl on wild-type and mutants | Escherichia coli |
additional information | - |
influence of KCl on wild-type and mutants | Acinetobacter calcoaceticus subsp. anitratus |
45 | - |
purified wild-type enzyme | Escherichia coli |
57 | - |
purified recombinant chimeric Acinetobacter-type protein with small E. coli domain | Acinetobacter calcoaceticus subsp. anitratus |
88 | - |
purified wild-type enzyme | Acinetobacter calcoaceticus subsp. anitratus |
120 | - |
purified recombinant chimeric E. coli-type protein with small Acinetobacter domain | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Escherichia coli | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Acinetobacter calcoaceticus subsp. anitratus | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | recombinant chimeric enzymes, domain functions, subunit organisation | Escherichia coli |
More | recombinant chimeric enzymes, domain functions, subunit organisation | Acinetobacter calcoaceticus subsp. anitratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | chimeric mutants, overview | Escherichia coli | |
additional information | - |
additional information | chimeric mutants, overview | Acinetobacter calcoaceticus subsp. anitratus | |
78 | - |
acetyl-CoA | wild-type, + 0.1 M KCl | Acinetobacter calcoaceticus subsp. anitratus | |
81 | - |
acetyl-CoA | wild-type, + 0.1 M KCl | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
recombinant chimeric protein | Escherichia coli |