Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, from 2-2.3 M ammonium sulfate, 2% v/v polyethylene glycol 400, 0.1 M HEPS, pH 6.0, X-ray diffraction analysis, structure determination and modeling: 3 identical dimer units arranged about a central 3-fold axis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | allosteric inhibition mechanism, structure relationship | Escherichia coli | |
NADH | allosteric; CS II, strong and specific allosteric inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABH7 | isozyme CS II | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | mechanism | Escherichia coli | |
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | active site | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Escherichia coli | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | subunit organization and crystal structure, amino acid residues involved in subunit interaction | Escherichia coli |