Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Streptomyces virginiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of EDTA (10 mM) to an assay not containing any divalent cations does not affect the enzyme activity | Streptomyces virginiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
geranyl diphosphate | with flaviolin as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
0.0027 | - |
geranyl diphosphate | with 1,3-dihydroxynaphthalene as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
0.021 | - |
flaviolin | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
1.9 | - |
1,3-dihydroxynaphthalene | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
5.6 | - |
4-hydroxybenzoate | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is independent of the presence of Mg2+ or other divalent metal ions | Streptomyces virginiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32700 | - |
1 * 32700, calculated from amino acid sequence | Streptomyces virginiae |
33200 | - |
1 * 33200, SDS-PAGE | Streptomyces virginiae |
39100 | - |
gel filtration | Streptomyces virginiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + flaviolin | Streptomyces virginiae | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | 3-linalylflaviolin + diphosphate | - |
? | |
geranyl diphosphate + flaviolin | Streptomyces virginiae DSM 1042 | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | 3-linalylflaviolin + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces virginiae | - |
- |
- |
Streptomyces virginiae DSM 1042 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose resin column chromatography, benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration | Streptomyces virginiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate + 1,3-dihydroxynaphthalene | - |
Streptomyces virginiae | 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol | - |
? | |
geranyl diphosphate + 1,3-dihydroxynaphthalene | - |
Streptomyces virginiae DSM 1042 | 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol | - |
? | |
geranyl diphosphate + 4-hydroxybenzoate | - |
Streptomyces virginiae | 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid | - |
? | |
geranyl diphosphate + 4-hydroxybenzoate | - |
Streptomyces virginiae DSM 1042 | 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid | - |
? | |
geranyl diphosphate + flaviolin | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | Streptomyces virginiae | 3-linalylflaviolin + diphosphate | - |
? | |
geranyl diphosphate + flaviolin | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | Streptomyces virginiae DSM 1042 | 3-linalylflaviolin + diphosphate | - |
? | |
additional information | does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate | Streptomyces virginiae | ? | - |
? | |
additional information | does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate | Streptomyces virginiae DSM 1042 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33200, SDS-PAGE | Streptomyces virginiae |
monomer | 1 * 32700, calculated from amino acid sequence | Streptomyces virginiae |
Synonyms | Comment | Organism |
---|---|---|
Fnq26 | - |
Streptomyces virginiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
flaviolin | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
0.002 | - |
4-hydroxybenzoate | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
0.003 | - |
1,3-dihydroxynaphthalene | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces virginiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.2 | half-maximal values at pH 7.5 and 9.2 | Streptomyces virginiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in FNQ I biosynthesis | Streptomyces virginiae |